Calcium bis[(Z)-N-methyl-N-(1-oxo-9-octadecenyl)aminoacetate]

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

6th February - 15th May 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

other: turbidity method

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: SOAP005-2
- Expiration date of the lot/batch: 15 October 2022

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient, stored under nitrogen in a desiccator

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Following receipt of the test item, it was found that the material readily absorbed moisture in the parent container. Consequently, on advice from the Sponsor, a sub-sample was dried overnight at 100°C and then stored in a desiccator under a headspace of nitrogen.

Water solubility:

45 mg/L

Conc. based on:

test mat.

Temp.:

20 °C

pH:

>= 5.4 - <= 7.1

Details on results:

The results were consistent with the visual observations on the samples, since at concentrations above the calculated solubility the solution were turbid.

Table 1 - Water solubility measurements (Sample A)

Point	Concentration (mg/L)	Mean turbidity (NTU)	pH	ln concentration	ln turbidity
1	1017	178	5.5	6.92	5.18
2	508.6	51.1	5.6	6.23	3.93
3	254.3	16.2	5.7	5.54	2.79
4	127.2	9.97	5.9	4.85	2.30
5	63.58	11.1	6.4	4.15	2.41
6^1	31.79	8.20	5.9	3.46	2.10
7	15.89	1.07	7.1	2.77	0.06
8	7.947	0.789	6.9	2.07	-0.24
9	3.973	0.485	6.8	1.38	-0.72
10	1.987	0.253	7.0	0.69	-1.37

¹Point 6 was excluded from the calculations as an outlier.

Linear regression of points 3 to 5, y = 0.272x + 1.18

Linear regression of points 5 to 9, y’ = 1.26x’ - 2.44

Table 2 - Water solubility measurements (Sample B)

Point	Concentration (mg/L)	Mean turbidity (NTU)	pH	ln concentration	ln turbidity
1	1045	167	5.4	6.95	5.12
2	522.6	54.1	5.4	6.26	3.99
3	261.3	18.6	5.5	5.57	2.92
4	130.7	11.6	5.5	4.87	2.45
5	65.33	15.3	5.5	4.18	2.73
6	32.66	8.38	5.6	3.49	2.13
7	16.33	3.25	5.8	2.79	1.18
8	8.166	1.27	5.7	2.10	0.24
9	4.083	0.562	6.8	1.41	-0.58
10	2.041	0.308	6.7	0.71	-1.18

Linear regression of points 3 to 5, y = 0.142x + 2.01

Linear regression of points 5 to 9, y’ = 1.21x’ - 2.18

Conclusions:

The water solubility of the test item was determined to be 45 mg/L at 20°C.

Executive summary:

The water solubility study with Calcium bis[(Z)-N-methyl-N-(1-oxo-9-octadecenyl)aminoacetate] was conducted according to the OECD guideline 105 using a turbidity method (Dunning 2018). A preliminary test was conducted to assess the approximate solubility of the test item and ascertain the appropriate test procedure for the definitive testing. A solubility of less than 10 mg/L was estimated. The test item is a surface active substance, therefore a turbidity method was selected for the determination of the water solubility of the substance. The test was conducted in duplicate in a temperature controlled environmental chamber at 20°C.

The water solubility of the test item was determined to be 45 mg/L at 20°C.

The study is GLP-compliant and was conducted according to the standard guideline, therefore is considered reliable without restrictions and suitable as a key study for this endpoint.

Description of key information

The water solubility of Calcium bis[(Z)-N-methyl-N-(1-oxo-9-octadecenyl) aminoacetate] is 45 mg/L at 20°C.

Key value for chemical safety assessment

Water solubility:

45 mg/L

at the temperature of:

20 °C

Additional information

The water solubility study was conducted according to the OECD guideline 105 using a turbidity method (Dunning 2018).

The water solubility of the test item was determined to be 45 mg/L at 20°C.

The study is GLP-compliant and was conducted according to the standard guideline, therefore is considered reliable without restrictions and suitable as a key study for this endpoint.