N-dodecyl-β-alanine, compound with 2,2',2''-nitrilotriethanol (1:1)

Biodegradation in soil

Administrative data

Endpoint:

biodegradation in soil: simulation testing

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance is readily biodegradable

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

Study performed on sodium salt.
The triethanolamine salt also dissociates and triethanolamine biodegradation has been well investigated

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

ca. 70

Sampling time:

9 d

Parameter:

% degradation (CO2 evolution)

Value:

ca. 96

Sampling time:

28 d

Details on results:

Two concentrations tested, but lower concentration (approx 10 mg/l C) gave a % degradation over over 100% and has been disregarded.
The higher concentration of approximately 30 mg/l C resulted in a plateau of about 100% degradation after day 22. The 10 day window was achieved.
The positive control group reached a plateau of about 80% after 22 days, but failed to reach the 60% in 10 day criteria.

Results with reference substance:

The positive control group reached a plateau of about 80% after 22 days, but failed to reach the 60% in 10 day criteria.

Validity criteria fulfilled:

no

Interpretation of results:

readily biodegradable

Executive summary:

There are obvious flaws in the testing, such as exceeding 100% for one of the concentrations tested and the poor performance of the positive control. However, earlier studies (albeit with less detail on methods and limited reporting) and estimations based on modelling and considering the class of substance suggests that the the substance is readily biodegradable.

Cross-reference 2

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data included to demonstrate that triethanolamine salt will be biodegradable; the presence of triethanolamine will not reduce the level of biodegradation observed for the sodium salt.
Peer reviewed journal using types of methods associated with guidelines.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Principles of method if other than guideline:

Various methods used, but the most useful results came from CO2 evolution measurements.
The publication also describes work using radiolabelled materials and examines kinetics of biodegradation

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Mixed liquor activated sludge (secondary effluent)

Duration of test (contact time):

5 d

Initial conc.:

ca. 40 mmol/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

not specified

Key result

Parameter:

% degradation (CO2 evolution)

Value:

ca. 100

Sampling time:

5 d

Details on results:

The purpose of the research was to measure kinetics and percentage rates of biodegradation are not reported for the activated sludge tests.
Other work reported in this publication suggest rates of > 60% in river water and natural soils.
The authors conclude that there is rapid biodegradation

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

The purpose of the research was to measure kinetics and percentage rates of biodegradation are not reported for the activated sludge tests.
Other work reported in this publication suggest rates of > 60% in river water and natural soils.
The authors conclude that there is rapid biodegradation