2-Naphthalenamine, N-(2-ethylhexyl)-1-[[4-(phenylazo)phenyl]azo]-, ar' and ar''-Me derivs.

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE report is based on biodegradation in water study.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : 2-Naphthalenamine, N-(2-ethylhexyl)-1-[[4-(phenylazo)phenyl]azo]-, ar' and ar''-Me derivs.
- IUPAC name : N-(2-ethylhexyl)-1-[4-(2-phenyldiazen-1-yl)phenyl]naphthalen-2-amine
- Molecular formula : C30H33N3
- Molecular weight : 435.60 g/mol
- Substance type : Organic
- Physical state : Liquid

Oxygen conditions:

not specified

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Concentration of sludge: 30ppm

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

not specified

Parameter followed for biodegradation estimation:

other: BOD and GC

Parameter followed for biodegradation estimation:

other: BOD and HPLC

Details on study design:

Standard type

Reference substance:

not specified

Key result

Parameter:

other: BOD

Value:

0.2

Sampling time:

14 d

Remarks on result:

other: 1. Not readily biodegradable

Key result

Parameter:

other: GC

Value:

0.3

Sampling time:

14 d

Remarks on result:

other: 1. Not readily biodegradable

Key result

Parameter:

other: BOD and HPLC

Value:

0

Sampling time:

28 d

Remarks on result:

other: Not readily biodegradable

Details on results:

1. 0.2 % degradation were observed by BOD and 0.3 % by GC
2. 0 % degradation were observed by BOD and HPLC parameter.

Validity criteria fulfilled:

not specified

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

1. The percentage degradation of test substance was determined to 0.2% by BOD and 0.3 % by GC parameter in 14 days. Thus, based on percentage degradation test chemical is considered to be not readily biodegradable in water.

2. The percentage degradation of test substance was determined to 0 % by BOD and 0 % by HPLC parameter in 28 days. Thus, based on percentage degradation of test chemical is considered to be not readily biodegradable in water.

Executive summary:

Biodegradation study was performed of the structurally and functionally similar read across chemicals. The studies are as mentioned below:

Study 1.

Biodegradation study was conducted for evaluating the percentage biodegradability of read across test substance. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 0.2% by BOD and 0.3 % by GC parameter in 14 days. Thus, based on percentage degradation test chemical is considered to be not readily biodegradable in water.

Study 2.

Similarly study was conducted on structurally and functionally similar read across chemical. Biodegradation study was conducted for evaluating the percentage biodegradability of test substance. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 0 % by BOD and 0 % by HPLC parameter in 28 days. Thus, based on percentage degradation of test chemical is considered to be not readily biodegradable in water. 

Thus based on both studies for structurally and functionally similar read across chemicals it can be concluded that the test chemical N-(2-ethylhexyl)-1-[4-(2-phenyldiazen-1-yl)phenyl]naphthalen -2-amine (92257-28 -8) was not readily biodegradable in water.

Description of key information

Biodegradation study was performed of the structurally and functionally similar read across chemicals. The studies are as mentioned below:

Study 1.

Biodegradation study was conducted for evaluating the percentage biodegradability of read across test substance. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 0.2% by BOD and 0.3 % by GC parameter in 14 days. Thus, based on percentage degradation test chemical is considered to be not readily biodegradable in water.

Study 2.

Similar study was conducted on structurally and functionally similar read across chemical. Biodegradation study was conducted for evaluating the percentage biodegradability of test substance. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 0 % by BOD and 0 % by HPLC parameter in 28 days. Thus, based on percentage degradation of test chemical is considered to be not readily biodegradable in water. 

Thus based on both studies for structurally and functionally similar read across chemicals it can be concluded that the test chemical N-(2-ethylhexyl)-1-[4-(2-phenyldiazen-1-yl)phenyl]naphthalen -2-amine (92257-28 -8) was not readily biodegradable in water.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

Various experimental data for the target compound N-(2-ethylhexyl)-1-[4-(2-phenyldiazen-1-yl)phenyl]naphthalen-2-amine (CAS No. 92257-28-8) and supporting weight of evidence studies for its

structurally and functionally similar closest read across substance were reviewed for the biodegradation end point which are summarized as below: 

 

Study 1.

Biodegradation study was conducted for evaluating the percentage biodegradability of read across test substance. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 0.2% by BOD and 0.3 % by GC parameter in 14 days. Thus, based on percentage degradation test chemical is considered to be not readily biodegradable in water.

Study 2.

Similarly study was conducted on structurally and functionally similar read across chemical. Biodegradation study was conducted for evaluating the percentage biodegradability of test substance. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 0 % by BOD and 0 % by HPLC parameter in 28 days. Thus, based on percentage degradation of test chemical is considered to be not readily biodegradable in water. 

On the basis of above results for target chemical N-(2-ethylhexyl)-1-[4-(2-phenyldiazen-1-yl)phenyl]naphthalen-2-amine (CAS No. 92257-28-8) and for its read across substance from authoritative database, it can be concluded that the test substance N-(2-ethylhexyl)-1-[4-(2-phenyldiazen-1-yl)phenyl] naphthalen-2-amine (CAS No. 92257-28-8) can be expected to be not readily biodegradable in nature.