Oxydiethylene bis(chloroformate)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Study of Industrial Bio-Test Laboratories (IBT).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

only 2 animal/sex and dose

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Oxydiethylene bis(chloroformate)

EC Number:

203-430-9

EC Name:

Oxydiethylene bis(chloroformate)

Cas Number:

106-75-2

Molecular formula:

C6H8Cl2O5

IUPAC Name:

oxydiethane-2,1-diyl dichlorocarbonate

Details on test material:

Purity of the test material was not indicated

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.44 - 2.90 kg
- food and water: ad libidum
- Acclimation period: at least 7 days

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24 h

Doses:

2.0, 3.0, 4.6, 6.8 g/kg bw

No. of animals per sex per dose:

2

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weighing prior to treatment and at 14 days (if still alive)
- Necropsy of survivors performed: on day 14

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

3 400 mg/kg bw

Mortality:

None of the animals treated with 2.0 g/kg died, but all weighed less at 14 days than at the beginning of the study. The two males treated with 3.0 g/kgand two males and 1 female treated with 4.6 g/kg died within 3 days oftreatment. Whereas the survivors treated with 3.0 mg/kg gained a slight amount of weight (50-100 g) over the 14 day observation period, the survivor treated with 4.6 mg/kg lost a slight amount of weight (80 g). All animals treated with 6.8 mg/kg died within 2 days of treatment.

Clinical signs:

No untoward behavioral reactions were noted. Pale red to red, well-defined erythema, moderate edema and burns were noted at the test site after 24 hours. Superficial escharosis was noted 6 days after treatment. The skin began to slough at 14 days

Body weight:

None of the animals treated with 2.0 g/kg died, but all weighed less at 14 days than at the beginning of the study. The two males treated with 3.0 g/kgand two males and 1 female treated with 4.6 g/kg died within 3 days oftreatment. Whereas the survivors treated with 3.0 mg/kg gained a slight amount of weight (50-100 g) over the 14 day observation period, the survivor treated with 4.6 mg/kg lost a slight amount of weight (80 g). All animals treated with 6.8 mg/kg died within 2 days of treatment.

Gross pathology:

No gross pathologic alterations other than the dermal alterations.

Any other information on results incl. tables

None of the animals treated with 2.0 g/kg died, but all weighed less at 14 days than at the beginning of the study. The two males treated with 3.0 g/kg and two males and 1 female treated with 4.6 g/kg died within 3 days of treatment. Whereas the survivors treated with 3.0 mg/kg gained a slight amount of weight (50-100 g) over the 14 day observation period, the survivor treated with 4.6 mg/kg lost a slight amount of weight (80 g). All animals treated with 6.8 mg/kg died within 2 days of treatment.

No untoward behavioral reactions were noted. Pale red to red, well-defined erythema, moderate edema and burns were noted at the test site after 24 hours.  Superficial escharosis was noted 6 days after treatment. The skin began to slough at 14 days. Necropsies of all animals did not reveal any gross pathologic alterations other than the dermal alterations.