Hexakis[μ-(acetato-O:O')]-μ3-oxo-triangulo-triruthenium acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Water samples taken at 0 and 48 hours for analysis

Vehicle:

no

Details on test solutions:

Preliminary range-finding study conducted at 0.010, 0.10, 1.0, 10 and 100mg/l.
In the definitive study, test material dissolved directly in water and ultrasonicated for 30 minutes, before being diluted to a 100 mg/L test concentration.

Test organisms (species):

Daphnia magna

Details on test organisms:

1st instar Dapnia magna from in-house laboratory cultures. Fed daily with a suspension of algae.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No data

Test temperature:

21°C

pH:

6.9-8.2

Dissolved oxygen:

8.1-8.4

Salinity:

Not applicable

Nominal and measured concentrations:

Limit test at a nominal concentration of 100mg/L. Measured concentrations close to nominal therefore EC50 value reported based on nominal concentrations.

Details on test conditions:

Test vessel: 250ml glass jars used and covered to reduce evaporation
Aeration: Test vessels were not aerated.
No of organisms per vessel: 10 daphnids per vessel
No of replicates per concentration: Four replicate test vessels prepared with duplicate control vessels
Photoperiod: 16h light: 8h dark
Feeding: No feeding during the exposure.

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobilisation in the 40 daphnids exposed.

Validity criteria fulfilled:

not specified

Conclusions:

The 48-hour EC50 for ruthenium acetate to Daphnia magna was determined to be >100 mg/L.

Executive summary:

This is a GLP compliant study following OECD guideline 202 and is considered suitable for use as the key study for this endpoint. Based on results from a preliminary test a limit test at 100 mg/L was conducted for the definitive study. Test concentrations were analysed and were close to nominal, therefore the EC50 value was reported based on nominal concentrations. The 48-hour EC50 for ruthenium acetate to Daphnia magna was determined to be >100 mg/L.

Description of key information

The 48-hour EC50 for ruthenium acetate to Daphnia magna was determined to be >100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Wetton and Mulle (2001) is a GLP compliant study following OECD guideline 202 and is considered suitable for use as the key study for this endpoint. Based on results from a preliminary test a limit test at 100 mg/L was conducted for the definitive study. Test concentrations were analysed and were close to nominal, therefore the EC50 value was reported based on nominal concentrations. The 48-hour EC50 for ruthenium acetate to Daphnia magna was determined to be >100 mg/L.