Hexakis[μ-(acetato-O:O')]-μ3-oxo-triangulo-triruthenium acetate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hexakis[μ-(acetato-O:O')]-μ3-oxo-triangulo-triruthenium acetate

EC Number:

259-653-7

EC Name:

Hexakis[μ-(acetato-O:O')]-μ3-oxo-triangulo-triruthenium acetate

Cas Number:

55466-76-7

Molecular formula:

C12H18O13Ru3.C2H3O2

IUPAC Name:

hexamethyl-2λ³-oxa-4λ³-oxa-6λ³-oxa-8λ³-oxa-10λ³-oxa-12λ³-oxa-13λ³-oxa-15λ³-oxa-16λ³-oxa-18λ³-oxa-19λ³-oxa-21λ³,22λ¹-dioxa-1,5,9-triruthenahexacyclo[7.3.3.3¹,⁵.3⁵,⁹.1¹,⁵.0⁹,²²]docosa-3,7,11,14,17,20-hexaene-1,1,1,5,5,5,9,9,9-nonakis(ylium)-2,6,10,13,16,19-hexaide-22,22-diuide acetate

Details on test material:

ruthenium acetate

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

Mixed population of sewage treatment microorganisms from second stage sewage treatment at Belper, Derbyshire.
The effluent was allowed to settle for approximately 1 hour, the supernatant removed and maintained on continuous aeration at a temperature of
21°C prior to use.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

12 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Stock solution concentration was 200 mg/l. Test concentration was 12 mg/l.
BOD bottles were set up as follows:
- Control (in duplicate)
- Reference material (in duplicate)
- Test material (in duplicate)
- Toxicity control (in duplicate)

Media was inoculated with sewage treatment micro-organisms at a rate of 1 drop of inoculum per litre.

BOD bottles were incubated in a water bath at 21°C.

Sampling for dissolved oxygen was performed on days 0, 3, 6, 9, 12, 15, 18, 21, 24 and 28.

Reference substance

Reference substance:

other: sodium benzoate

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

59

Sampling time:

28 d

Details on results:

Toxicity control containing both Ruthenium acetate and sodium benzoate, attained 51% degradation after 28 days thereby confirm that the test material was not toxic to the sewage treatment micro-organisms used in the study

BOD5 / COD results

Results with reference substance:

Sodium benzoate (3mg/l) with inoculum attained 81% degradation in 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Ruthenium acetate attained 59% degradation after 28 days and therefore cannot be considered as readily biodegradable under guideline 301D.

Executive summary:

This is a GLP compliant study following OECD guideline 301D. A control, toxicity control and reference item were all included in the study set up. The toxicity control containing both ruthenium acetate and sodium benzoate, attained 51% degradation after 28 days thereby confirms that the test material was not toxic to the sewage treatment micro-organisms used in the study The study is considered to be suitable for use as the key study for this endpoint. Ruthenium acetate attained 59% degradation after 28 days and therefore cannot be considered as readily biodegradable under guideline 301D.