Biphenyl-2-yl-(9,9-dimethyl-9H-fluoren-2-yl)-(9,9’-spirobifluoren-2-yl)-amine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Aug 18, 2014 - Nov 11, 2014

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No OECD GLP

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Appearance: white to pale yellow powder
Water solubility: < 0.005 mg/L (20 °C)

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

The test substance was dissolved in the test solution, which was prepared each 48 hours.
The concentration was 100 mg/L. After 30 min ultrasound treatment, the turbide mixture was stirred for 48 hours, and then filtered with a 0.45 µm membrane filter to prepare the test solution.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Strain: Dania rerio
- Source: Hangzhou Minghong Aquarium
- Received on: 2014-02-20
- Batch: FZA000-20140220-03
- Length at study initiation: 2.12-2.99 cm
- Weight at study initiation: 2.64-2.78 g
- Method of breeding: standard
- Feeding during test: tetra min diet during acclimatization, with daphnia or artemia, or frozen mosquito larva daily

ACCLIMATION
- Acclimation period: more than 7 days
- Acclimation conditions (same as test or not): same
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

test solution renewal after 48 h

Post exposure observation period:

no

Hardness:

45 mg CaCO3 / L

Test temperature:

23.3 -23.9 °C

pH:

6.9-7.5

Dissolved oxygen:

60.8 - 98.6 %

Salinity:

no data

Conductivity:

no data

Nominal and measured concentrations:

100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 5 L
- Type: open
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: light : dark = 14 h /10 h

EFFECT PARAMETERS MEASURED:
Clinical Examination: daily
(loss of equilibrium, changes in swimming behavior, respiratory function, pigmentation, dead)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: -
- Test concentrations: 100 mg/l
- Results used to determine the conditions for the definitive study: pretest

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no

Validity criteria fulfilled:

yes

Conclusions:

No aquatic toxicity was observed in this test system up to a nominal concentration of 100 mg/L test material. The 96h LC50 and the 96h NOEC were above the water solubility limit and larger than 100 mg/L (nominal).

Description of key information

The 96h LC50 value obtained from a study according to OECD Guideline 203 was above the water solubility limit and larger than 100 mg/L (nominal).