Propan-2-yl N-[(S)-(pentafluorophenoxy)(phenoxy) phosphoryl]-L-alaninate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 November 2012-14 January 2013

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Propan-2-yl N-[(S)-(pentafluorophenoxy)(phenoxy)-phosphoryl]-L-alanine

EC Number:

695-076-0

Cas Number:

1334513-02-8

Molecular formula:

C18H17F5NO5P

IUPAC Name:

Propan-2-yl N-[(S)-(pentafluorophenoxy)(phenoxy)-phosphoryl]-L-alanine

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Test item
- Identification: GS-606962
- Description: White powder
- Substance type: organic
- Lot/batch No.: PSI-172(2)-1-1-12001
- Storage condition of test material: room temperature in the dark

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.23 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

other: 100% v/v saturated solution

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.23 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

other: 100% v/v saturated solution

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test itemto the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EC50 of greater than 0.23 mg/L based on time weighted mean measured test concentrations. The No Observed Concentration was 0.23 mg/L based on time weighted mean test concentrations.
This study showed no effects at saturation.

Executive summary:

Introduction

A study was performed to assess the acute toxicity of the test item to Daphnia magna.

The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No. 202, "Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.

Methods

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at a concentration of 100% v/v saturated solution for 48 hours at a temperature of 20°C to 22°C under semi-static test conditions. The test item solution was prepared by stirring an excess (50 mg/L) of test item in test medium using a propeller stirrer at approximately 1500 rpm for 24 hours. After the stirring period any undissolved test item was removed by filtration (0.2 µm Sartorius Sartopore filter, first approximate 2 liters discarded in order to pre-condition the filter) to produce a 100% v/v saturated solution of the test item. Immobilization and any adverse reactions to exposure were recorded after 24 and 48 hours.

Results

Chemical analysis of the freshly prepared 100% v/v saturated solution test preparation at 0 hours showed that a measured concentration of 0.275 mg/L and at 24 hours showed that a measured test concentration of 0.362 mg/L was obtained. A decline in measured test concentration was observed in the old or expired test media at 24 hours to 0.152 mg/L and at 48 hours to 0.105 mg/L. This decline was considered to be due to the unstable nature of the test item. Given this decline in measured test concentration it was considered justifiable to base the results on the time weighted mean test concentrations.

The 48 -Hour EC₅₀ for the test item to Daphnia magna based on the time weighted mean test concentrations was greater than 0.23 mg/L. The No Observed Concentration was 0.23 mg/L based on time weighted mean test concentrations.

This study showed no effects at saturation.