1-Hepten-4-yn-3-ol, 6,6-dimethyl-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

6,6-dimethylhept-1-en-4-yn-3-ol

EC Number:

616-644-6

Cas Number:

78629-20-6

Molecular formula:

C9H14O

IUPAC Name:

6,6-dimethylhept-1-en-4-yn-3-ol

Test material form:

liquid

Details on test material:

Test item: enynolCAS No.: 78629-20-6Batch number: L62042NPhysical state: colourless liquidStorage: store it in a cool, dry, well-ventillated place, free from direct sunlight

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

Preparation of stock solution:
The preparation of stock and test solutions was based on the preliminary non GLP range finding test. As the Range Finding Test was not performed in compliance with the GLP-Regulations it is excluded from the Statement of Compliance in the final report, but the raw data of this test are archived under the study code of present study. For the stock solution 100.11 mg of test item was measured into 1 000 ml Elendt M4 medium. The suspension was stirred till complete dissolution. After stirring the stock solution was clear and colourless. The nominal concentration of stock solution was 100mg/L. The measured concentration of the stock solution was 95.55 mg/L.

Preparation of test solutions:
Seven dilution levels were prepared by mixing appropriate volume of Elendt M4 medium and stock solution of the test item. The maximum separation factor between of test vessels was 2. The following table shows the preparation of the different dilution levels. The volume of the prepared test solutions was 300 ml.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test species: Daphnia magna Straus
Age: < 24 hours neonates
Origin: National Institute of Environmental Health
Date of arriving: 16 May 2013
Culture conditions: The test species is maintained in Elendt M4 medium with culture conditions at 21±1 °C and a light/dark cycle of 16/8 hours with light intensities of less than 1000 Lux. The cultures are fed with centrifugalized green alga (Pseudokirchneriella subcapitata) cells daily at weekdays.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

The temperature of thermostat was recorded daily and registered continuously by Extech datalogger. The temperature inside the thermostat at the test vessels was measured and registered continuously by Extech SD200 thermometer and datalogger.

pH:

The pH was measured at the beginning and at the end of the test. At the beginning of the test the pH was measured from the remaining test solutions of the concurrent test vessels at each dilution levels. At the end of the test the pH was measured in each test vessel.

Dissolved oxygen:

The dissolved oxygen was measured at the beginning and at the end of the test. At the beginning of the test the dissolved oxygen was measured from the remaining test solutions of the concurrent test vessels at each dilution levels. At the end of the test the dissolved oxygen was measured in each test vessel.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

8.01 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

7.19 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

other: EC20

Effect conc.:

7.46 mg/L

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Remarks on result:

not determinable

Effect concentrations 5

Duration:

48 h

Dose descriptor:

LOEC

Remarks on result:

not determinable

Details on results:

The No Observed Effect Concentration (NOEC) and Lowest Observed Effect Concentration (LOEC) of the test item were determined with Fisher’s exact binominal test with Bonferroni correction by ToxRat® software. The results were not applicable tothe data obtained.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Acute toxic effect of Enynol was investigated in Daphnia sp. Acute Immobilisation test (according to OECD 202 guideline). The EC50 was 8.01 mg/L at 48 h.
In this Daphnia sp., Acute Immobilisation Test for Enynol the results indicated that the test item is hazardous to the aquatic environment and classified into the second acute toxic hazardous category [GHS Acute aquatic hazard Category Acute II. – 48 hr EC50 (for Crustacea) > 1 mg/L but ≤ 10 mg/L]. As the lowest test concentration of the test item at the end of the test was out of the concentration range of the validated analytical method, the calculated acute toxic effect toward Daphnia magna Straus must be interpreted by precaution.