Styrene

Toxicity to reproduction: other studies

Administrative data

Endpoint:

toxicity to reproduction: other studies

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented publication meeting basic scientific principles. Investigation of styrene and styrene oligomers.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Endocrine-disrupting effects of styrene oligomers that migrated from polystyrene containers into food.

Author:

Date, K. et al.

Year:

2002

Bibliographic source:

Food and Chemical Toxicology 40: 65-75

Reference 2

Reference Type:

review article or handbook

Title:

European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.

Author:

European Union

Year:

2007

Bibliographic source:

Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007

Materials and methods

Principles of method if other than guideline:

In this study, the endocrine-disrupting effects of styrene monomer, styrene dimer and styrene trimer were evaluated by the androgen receptor binding assay.

GLP compliance:

not specified

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

Styrene

EC Number:

202-851-5

EC Name:

Styrene

Cas Number:

100-42-5

Molecular formula:

C8H8

IUPAC Name:

ethenylbenzene

Specific details on test material used for the study:

- Name of test material (as cited in study report): styrene monomer (styrene dimer and styrene trimer)
- Analytical purity: 99%

Test animals

Species:

rat

Strain:

other: Crj:CD (SD) IGS rats

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Laboratories (Hino, Japan)
- Age at study initiation: 8 week-old
- Weight at study initiation: 260-300 g
- Diet (e.g. ad libitum): Charles River Certified rodent diet (CRF-1); ad libitum
- Water (e.g. ad libitum): water; ad libitum
- Other: The animals were selected on the basis of body weight and freedom from clinical signs of disease or injury during the quarantine period.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23.5 ± 2.0
- Humidity (%): 55 ± 10
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

other: androgen receptor binding assay in vitro

Vehicle:

DMSO

Analytical verification of doses or concentrations:

not specified

Doses / concentrations

Remarks:

Doses / Concentrations:
0.1 nmol/L - 10 µmol/L
Basis:
nominal conc.

No. of animals per sex per dose:

not applicable

Control animals:

other: AR binding assay in vitro

Statistics:

Data were expressed as mean±standard deviation. The statistical significance between multiple groups was analysed by one-way analysis of variance (ANOVA). When the F test was significant, it was followed by Dunnett´s multiple comparison test. The significance level for all tests was set at P<0.05.

Results and discussion

Effect levels

Dose descriptor:

NOAEL

Effect level:

> 10 other: µmol/L

Sex:

male

Basis for effect level:

other: AR binding assay in vitro

Observed effects

Styrene monomer, styrene dimer or styrene trimer did not inhibit the binding of [3H]R1881 to AR at the concentration ranging from 0.1 nmol/L to 10 µmol/L.
DHT inhibited the binding of [3H]R1881 to AR in a dose-dependent manner, the IC50 value was 4.21 nmol/L.