Pent-1-ene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented study but not performed under GLP. Testguideline similar to OECD test guideline, but the organism E.Coli was not tested. Substance purity not reported.

Data source

Reference

Reference Type:

other: Study result available in the National Toxicology Program database

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: NTP Standard Protocol

Deviations:

yes

Remarks:

The test was not conducted with E. Coli

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

The test was not conducted with E. Coli

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Pent-1-ene

EC Number:

203-694-5

EC Name:

Pent-1-ene

Cas Number:

109-67-1

Molecular formula:

C5H10

IUPAC Name:

pent-1-ene

Details on test material:

No indication on the purity of the test substance was reported.

Method

Target gene:

histidine gene

Species / strain

Species / strain / cell type:

bacteria, other: S. Typhimurium TA97, TA98, TA100, TA1535

Metabolic activation:

with and without

Metabolic activation system:

metabolic activation enzymes and cofactors from Aroclor 1254-induced male Sprague-Dawley rat or Syrian hamster liver S9 (10 and 30%)

Test concentrations with justification for top dose:

0, 100, 333, 1000, 3333, 10000 ug/plate

Vehicle / solvent:

Dimethyl Sulfoxide

Controls

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: See Details on test system and conditions

Details on test system and experimental conditions:

Positive control chemicals used in NTP Ames tests:

For strains tested in the absence of S9:
- TA98, 2-nitrofluorene or alternatively 4-nitro-o-phenylenediamine
- TA100 and TA1535, sodium azide
- TA97, 9-aminoacridine

For strains tested with S9:
- All strains, 2-aminoanthracene (or occasionally, sterigmatocystin)

METHOD OF APPLICATION:
preincubation

DURATION
- Preincubation period: 20 minutes
- Exposure duration: 2 days

Results and discussion

Test results

Species / strain:

S. typhimurium, other: S. typhimurium TA 1535, TA 97, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Remarks:

only slight cytotoxicity was measured for 10000 ug/plate without HLI

Vehicle controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

See attachment in section "Attached background material".

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

According to this test, no mutagenic activity of 1-Pentene was found.

Executive summary:

Mutagenicity was tested on four S. typhimurium strains according to the NTP standard protocol (Ames test). 1-Pentene was not found to be mutagenic to bacteria, with and without metabolic activation.