Benzenepropanoic acid, .beta.,4-dimethoxy-, methyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study conducted in accordance with GLP, however the test substance had a very low purity (e.g. < 90 %).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EU Directive 79/831/EEC, Annex V, part C.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl 3-methoxy-3-(4-methoxyphenyl)propanoate

EC Number:

614-925-8

Cas Number:

69098-08-4

Molecular formula:

C12 H16 O4

IUPAC Name:

methyl 3-methoxy-3-(4-methoxyphenyl)propanoate

Details on test material:

- Name of test material (as cited in study report): 3- (4-Methoxyphenyl) -3-methoxypropionsaeure methylester, techn.
(Chemical name: Benzenepropanoic acid, -beta-, 4-dimethoxy-,
methyl ester)
- Lot/batch No.: Test dilution 165 (manufactured on 22 Apr 1993)
- Analytical purity: 80.7 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A defined amount of the substance was weighed out and suspended directly in the test medium by stirring (about 20 h at 20 °C) and then separated by centrifugation (6000 rpm for 20 minutes). The nominal concentration of the stock solution was 10 g/l. By dilution of this stock solution with test medium the nominal concentrations were prepared.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 +/- 2 °C

Nominal and measured concentrations:

Nominal test concentrations:
control, 1.31, 2.63, 5.25, 10.5, 21, 42, 84, 168 and 336 mg/l.

Details on test conditions:

TEST SYSTEM
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1/2 test organism/ml


OTHER TEST CONDITIONS
- Photoperiod: artificial light, 16 : 8 hours day-night regime
- Light intensity:about 5 - 6 uE/(m2/s) in the range of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

42 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

106.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

336 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

The EC values are given in mg/l (nominal concentration)

Time [h]/ EC0 / EC50  /    CL 95 %    / EC 100 /
3       / 168 / 249.7 / 213.6 - 291.8 /  > 336  /
24      /  84 / 136.5 / 120.5 - 154.6 /  336   /
48      /  42 / 106.5 / 92.4 - 122.7  /  336   /