6,10-dimethylundeca-3,5,9-trien-2-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, however a solubiliser was used

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, L11

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6,10-dimethylundeca-3,5,9-trien-2-one

EC Number:

205-457-1

EC Name:

6,10-dimethylundeca-3,5,9-trien-2-one

Cas Number:

141-10-6

Molecular formula:

C13H20O

IUPAC Name:

6,10-dimethylundeca-3,5,9-trien-2-one

Details on test material:

- Name of test material (as cited in study report): 2-Pseudoionon

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution with a nominal concentration of 100 mg/l was prepared. Following this, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. Chremophor was used as a solubiliser.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Bundesgesundheitsamt, Berlin, Germany
- Method of breeding: Single individuals in glass vessels (100 ml ) filled with 70 ml water

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

21 °C

pH:

7.52 - 7.75

Dissolved oxygen:

8.0 - 8.4 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.58,
1.0,
1.8,
3.2,
5.8,
10,
control,
solvent control

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessels (50 ml)
-Fill volume: 20 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412, part 11, 8.2
- Ca/mg ratio: 5:1
- Conductivity: 658 µS/cm

OTHER TEST CONDITIONS
- Light intensity: diffuse light (10 µE/qm*s)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 3, 6, 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 2.9 - 4.1 mg/l 95 % CL

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

EC0 (24 h) = 1.8 mg/l
EC50 (24 h) = 4.9 mg/l
EC100 (24 h) = 10 mg/l
Cumulative immobility of Daphnia in solvent control after 24 and 48 h: 5 %

Results with reference substance (positive control):

(24 h)
EC0 (24 h) = 0.58 mg/l
EC50 (24 h) = 1.29 mg/l
EC100 (24 h) = 3.2 mg/l