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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Toxicity to reproduction: other studies

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Administrative data

Endpoint:
toxicity to reproduction: other studies
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
in or before 2006
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
abstract

Data source

Reference
Reference Type:
publication
Title:
Effect of diethyl maleate induced oxidative stress on male reproductive activity in mice: redox active enzymes and transcription factors expression
Author:
Kaur P
Year:
2006
Bibliographic source:
Mol Cell Biochem. 2006, Oct; 291(1-2):55-61

Materials and methods

Principles of method if other than guideline:
evaluation of oxidative stress
GLP compliance:
not specified
Type of method:
in vivo

Test material

Constituent 1
Chemical structure
Reference substance name:
Diethyl maleate
EC Number:
205-451-9
EC Name:
Diethyl maleate
Cas Number:
141-05-9
Molecular formula:
C8H12O4
IUPAC Name:
diethyl (Z)-but-2-enedioate
Details on test material:
no details on test material available

Test animals

Species:
mouse
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
no data

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
not specified
Details on exposure:
Intraperitoneal injection of the test substance to males for two weeks
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
2 weeks
Frequency of treatment:
no data
Duration of test:
no data
No. of animals per sex per dose:
no data
Details on study design:
no data
Statistics:
no data

Results and discussion

Observed effects

Sperm concentration and motility were found to be significantly reduced along with a significant reduction in the litter size.

Applicant's summary and conclusion

Conclusions:
After intraperitoneal injections of the test substance to male mice for 2 weeks sperm concentration and motility were found to be significantly reduced along with a significant reduction in the litter size.
Executive summary:

Free radicals and reactive oxygen species (ROS) associated with oxidative stress are likely to play a number of significant roles in male reproduction. Present study was carried out to evaluate the effect of diethyl maleate (DEM) induced oxidative stress on male fertility in mice. Intraperitoneal injection of DEM for two weeks resulted in decrease in reduced glutathione and increase in the oxidized glutathione levels in the testis. Effect on the reproductive ability in term of sperm concentration, motility and fertility status was studied. Sperm concentration and motility were found to be significantly reduced along with a significant reduction in the litter size. Expression of redox sensitive transcription factor, cjun and cfos genes, along with gammaglutamyl cysteine synthetase (GCS) and manganese superoxide dismutase (MnSOD) expression were also studied using RT-PCR after DEM treatment. RT-PCR analysis revealed decrease in the testicular mRNA expression for cjun and cfos whereas the expression for GCS and MnSOD increased. Enzyme activity of SOD also increased. The study reflects the effect of DEM induced oxidative stress on the reproductive ability of male mice and interplay of the various components of the antioxidant defense system and redox regulated transcription factors at the transcriptional level.