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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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Ecotoxicological information

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
3 Hours
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Test material supplied to the laboratory as ca 50% commercial grade mixture in ethanediol and butanol with water.
The presence of these solvents were not considered to impact on the test result.
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Test material supplied to the laboratory as ca 50% commercial grade mixture in ethanediol and butanol with water.
The presence of these solvents were not considered to impact on the test result.
Analytical monitoring:
not required
Vehicle:
no
Test organisms (species):
activated sludge of a predominantly domestic sewage
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
21 °C - 22 °C
pH:
7 - 8
Nominal and measured concentrations:
10, 100 and 1000 mg/L (three replicates of the 1000 mg/L test concentration) as test material
5, 50 and 500 mg/l expressed as actives (ca 50% mixture tested)
Reference substance (positive control):
yes
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 50 - < 500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Duration:
3 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition
Details on results:
At the highest tested concentration (1000 mg/l of the 50% test matrial as received), there was 60 - 70% inhibtion and at 100 mg/l test material (50 mg/l actives), there was less than 50%.
Converting to the active ingredient, the IC50 is in the range of 50 - 500 mg/l
Validity criteria fulfilled:
yes
Conclusions:
Although the top concentration tested was only ca 500 mg/l actives (1000 mg/l as 50% solution), the observation of inhibition at the highest concentration allows an approximate IC50 to be calculated.
Test material supplied to the laboratory as ca 50% commercial grade mixture in ethanediol and butanol with water.
The presence of these solvents were not considered to impact on the test result.

Description of key information

Although the top concentration tested was only ca 500 mg/l actives (1000 mg/l as 50% solution), the observation of inhibition at the highest concentration allows an approximate IC50 to be calculated.

Test material supplied to the laboratory as ca 50% commercial grade mixture in ethanediol and butanol with water.

The presence of these solvents were not considered to impact on the test result.

Key value for chemical safety assessment

EC50 for microorganisms:
100 mg/L
EC10 or NOEC for microorganisms:
50 mg/L

Additional information

A precise IC50 was not calculated, but is between 50 and 500 mg/l. A figure of 100 mg/l is used for the CSR