Fatty acids, tall-oil, compds. with (Z)-N-9-octadecenyl-1,3-propanediamine (2:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Testing was conducted between 19 January 2010 and 10 March 2010.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

adopted July 17, 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Additiv 309

IUPAC Name:

Additiv 309

Details on test material:

Sponsor's identification: Additiv 309
Description : limpid brown, pasty liquid
Batch number : 651086751
Expiry date : 18 December 2013
Storage conditions: room temperature, protected from moisture and light

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Remarks:

(aqueous phase)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from the sewage plant at Hildesheim is well suited as it receives predominantly municipal sewage and hardly industrial chemical waste.
- Pretreatment: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was filled up with mineral salts medium and was maintained in an aerobic condition by aeration for 4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition by aeration with CO2 free air for 2 days. 10 mL/L were used to initiate inoculation.
- Colony forming units in the test vessel: 10E7 - 10E8 CFU/L

Duration of test (contact time):

47 d

Initial test substance concentration

Initial conc.:

13 mg/L

Based on:

other: carbon concentration

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: Mineral salts medium acc. to OECD 301 B / C02 Evolution Test
- Test temperature: 20.0 - 23.5 °C
- pH: ca. 7.7 (pH of all solutions was only messured once on day 47 prior to acidification)
- Aeration: 30 - 100 mL/min
- Continuous darkness: no, but low light conditions

TEST SYSTEM
- Culturing apparatus: test vessel: 5000 mL, brown glass; volume of the test medium: 3000 mL; dispersion treatment: continuous stirring
- Number of culture flasks/concentration: The following incubation vessels were prepared:
• two for the test item (P1, P2)
• one for the reference item (R1)
• two for the inoculum control (C1, C2)
• one for the toxicity control (T1)
- Measuring equipment:
pH-Meter, Multi 350i, WTW
Thermohygrograph, type 3.015/3 K, fabr.-no. 9003146
Flow meter, Typ DK 800 PV, KROHNE DUISBURG
Ultrasonic bath, SONOREX, BANDELIN
Magnetic stirrer, VARIOMAG
Carbon analyser, ANALYTIK JENA
Analytical balance, SARTORIUS
Balance, EW 3000-2M, KERN

CONTROL AND BLANK SYSTEM
- Inoculum blank: Test medium without test and/or reference item (duplicates)
- Toxicity control: Test item and reference item in test concentration (single)

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

56

Sampling time:

47 d

Remarks on result:

other: mean value of two determinations

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

36

Sampling time:

28 d

Remarks on result:

other: mean value of two determinations

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item must be regarded as not readily biodegradable in the 10-d-window and after 47 days.

Executive summary:

The ready biodegradability of the test item was determined with a non adapted activated sludge over a test period of 47 days in the Modified Sturm Test. The study was conducted from 2010-01-19 to 2010-03-10 according to OECD 301B (GLP). The test item was tested corresponding to a carbon concentration of 13 mg C/L in duplicates. The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO₂ produced by the respiration of bacteria. The degradation was stopped on day 47 by acidification of the test solutions. The last titration was made on day 48, after residual CO₂ had been purged from the test solutions over a period of 24 h. The percentage CO₂ production was calculated in relation to the theoretical CO₂ production (ThCO₂) of the test item. The biodegradation rate was calculated for each titration time. To check the activity of the test system, sodium benzoate was used as functional control. The percentage degradation of the functional control reached the pass level of 60 % after 6 days and came to 100 % after 35 days. In the toxicity control containing both test and reference item a biodegradation rate of 46 % was determined within 14 days and it came to 71 % after 47 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control. The test item reached the 10 % level (beginning of biodegradation) after 10 days. In the 10-d-window a mean biodegradation rate of 28 % was reached. The 1st test item replicate did not reach the 60 % pass level, after 47 days a biodegradation rate of 43 % was reached. The 2nd test item replicate reached the pass level of 60 % after 46 days and came to 69 % on day 47. The mean biodegradation rate after 47 days was 56 %. In conclusion, especially the biodegradation kinetics of the 2nd test replicate revealed that the test item is potentially inherently biodegradable.

Biodegradation of the test item in comparison to the functional control and toxicity control:

	Biodegradation [%]  Study Day [d]
	6	14	21	28	35	42	47
Test item, 1st replicate 13 mg C/L	3	20	28	34	40	40	43
Test item, 2nd replicate 13 mg C/L	3	20	30	38	48	55	69
Functional control 20 mg/L	61	80	87	96	100	100	100
Toxicity control 13 mg C/L test item + 20 mg/L reference item	26	46	54	60	65	66	71