Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-698-3 | CAS number: 4454-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline with acceptable restrictions (only 4 strains tested).
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
- Reference Type:
- secondary source
- Title:
- 3,4-Dihydro-2-methoxy-2H-pyran (CAS No. 4454-05-1)
- Author:
- OECD
- Year:
- 2 003
- Bibliographic source:
- cited in OECD SIDS 3,4-Dihydro-2-methoxy-2H-pyran for SIAM 16, 27-30 May 2003, Paris, France.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Only 4 strains tested
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,4-dihydro-2-methoxy-2H-pyran
- EC Number:
- 224-698-3
- EC Name:
- 3,4-dihydro-2-methoxy-2H-pyran
- Cas Number:
- 4454-05-1
- Molecular formula:
- C6H10O2
- IUPAC Name:
- 2-methoxy-3,4-dihydro-2H-pyran
- Details on test material:
- - Name of test material (as cited in study report): 2-Methoxi-2,3-dihydro-4H-pyran
- Analytical purity: >99.9%
- Impurities (identity and concentrations): not reported
- Lot/batch No.: not reported
- Storage condition of test material: Room temperature
Constituent 1
Method
- Target gene:
- Salmonella typhimurium: histidine operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S-9 mix
- Test concentrations with justification for top dose:
- Experiment 1: 0, 20, 100, 500, 2500 and 5000 µg/plate for all strains;
Experiment 2: 0, 100, 500, 2500, 5000 and 7500 µg/plate for TA100 - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene for all strains
- Remarks:
- with S9 mix
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without S9 mix Migrated to IUCLID6: for TA 1537
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N-nitro-N-nitrosoguanidine for TA100 and TA1535
- Remarks:
- without S9 mix
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylendiamine for TA98
- Remarks:
- without S9 mix
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48-72 hr at 37°C
NUMBER OF REPLICATIONS: 3 - Evaluation criteria:
- - doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results - Statistics:
- Mean and standard deviation calculated in result tables. No further data.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Remarks:
- from 500 µg/plate onwards
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: the test substance was completely soluble in DMSO - Remarks on result:
- other: other: S. typhimurium TA 1535, TA 1537, TA 98
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Mean reverants/plate
Experiment 1
Dose (µg/ml) |
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
||||
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
|
0 |
22 |
35 |
100 |
122 |
16 |
18 |
8 |
8 |
20 |
23 |
31 |
101 |
123 |
12 |
22 |
8 |
9 |
100 |
24 |
30 |
106 |
150 |
18 |
21 |
7 |
8 |
500 |
24 |
42 |
105 |
218 |
18 |
19 |
7 |
7 |
2500 |
22 |
33 |
119 |
315 |
15 |
22 |
7 |
9 |
5000 |
22 |
37 |
124 |
418 |
16 |
26 |
11 |
16 |
Positive control |
1163 |
1267 |
1025 |
1743 |
775 |
160 |
547 |
180 |
Experiment 2
Dose (µg/ml) |
TA 100 |
|
-S9 |
+S9 |
|
0 |
104 |
101 |
100 |
94 |
115 |
500 |
103 |
133 |
2500 |
113 |
272 |
5000 |
120 |
331 |
7500 |
127 |
436 |
Positive control |
903 |
1477 |
Applicant's summary and conclusion
- Executive summary:
This study was conducted comparable to the OECD Guideline 471 and is reliable with acceptable restrictions (only 4 strains tested). The test substance was tested in the standard plate test with and without metabolic activation (MA) in S. typhimurium TA98, TA100, TA1535 and TA1537 at dose levels of 20 -7500 µg/plate. Significant increase in the number of revertants was detected in TA100 with MA. No increase in the number of revertants was detected in any other strain with and without MA. No bacteriotoxic effect was observed. Vehicle controls and positive controls were valid.
Conclusion: According to the results of the present study, the test substance is mutagenic in the Salmonella typhimurium reverse mutation assay under the experimental conditions chosen here.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.