Dinaphtho[1,2,3-cd:3',2',1'-lm]perylene-5,10-dione, (9,10-dihydro-9,10-dioxo-1-anthracenyl)amino derivs.

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

COD determination

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 December, 1995 to 18 January, 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: DIN 38409- H 41-1 (1980) corresponding to EEC Directive 92/69/ C.6. (1992).

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

Principles of method if other than guideline:

Guideline followed

GLP compliance:

yes

Specific details on test material used for the study:

- Code number: FAT 45082/G
- Batch-Nr.: Op. 11.56
- Purity: ca. 85 %
- Appearance: solid, black
- Solubility: insoluble [in water]
- Storage: room temperature
- Expiration date: 10/1999

Oxygen conditions:

not specified

Inoculum or test system:

not specified

Details on inoculum:

None

Duration of test (contact time):

2 h

Details on study design:

TEST CONDITIONS
- Test temperature:148 ± 3 °C
- Duration: 2 hours
- Estimation Technique: Titration (potentiometric), METROHM Titroprozessor 670 (SAV: 00 111)

Reference substance:

other: Potassium hydrogen phthalate solution (Merck-Nr. 11769)

Preliminary study:

None

Test performance:

None

Remarks on result:

not measured/tested

Details on results:

None

Key result

Parameter:

COD

Value:

1 789 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate is: 193 mg O2/L
(Validity: 200 ± 8 mg O2/L)

Validity criteria fulfilled:

yes

Interpretation of results:

other: COD

Conclusions:

The COD of FAT 45082/G was determined to be 1789 mg O2/g.

Executive summary:

The Chemical Oxygen Demand (COD) of FAT 45082/G was determined in a study conducted according to DIN 38409- H 41-1 (1980) corresponding to EEC Directive 92/69/ C.6. (1992). A predetermined amount of the test substance FAT 45082/G in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test substance was tested with a sample weight of 4.48 and 4.49 mg. The reference substance potassium hydrogen phthalate was tested with 20 mL of a 0.17 g/l solution. The COD of FAT 45082/G was determined to be 1789 mg O₂/g, while the COD of the reference substance was 193 mg O₂/l.

Reference 2

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05 February, 1997 to 11 April, 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Deviations:

yes

Remarks:

The test substance was run in duplicate instead of in triplicate. Abiotic control. Reference control

Principles of method if other than guideline:

Guideline followed

GLP compliance:

yes

Specific details on test material used for the study:

Test item: FAT 48052/G
Batch: OP 11.56
Appearance: solid black
Solubility: insoluble
Storage: room temperature
Expiry date: 10/1999
ThOD: 2.32 mg O2/mg (ThOD calculated without nitrification)

Oxygen conditions:

aerobic

Inoculum or test system:

other: Mixture of polyvalent bacteria collected on February 10, 1997 from the aeration tank of a domestic sewage treatment plant, PRO RHENO Basel communal.

Details on inoculum:

- Pretreatment: The sludge was washed 4 times with tap water and the last time with test medium. An amount corresponding to 5 g dry material per liter (±10 %) was mixed with test medium and aerated until use.
- Concentration of sludge: 100 mg/L suspended solids

Duration of test (contact time):

28 d

Details on study design:

TEST CONDITIONS
- Test temperature: 24 - 25.5 °C
- pH: 7.1

Reference substance:

other: Sodium benzoate powder, for foodstuffs (restricted use) [Merck No. 6290]

Preliminary study:

None

Test performance:

None

Key result

Parameter:

other: BOD versus ThOD

Value:

7

Sampling time:

28 d

Remarks on result:

other: Std deviation not specified

Details on results:

The mean value of the biodegradation of FAT 45082/G corrected by the blank control and measured as BOD versus ThOD was : 7 % after 28 days
The BOD5 after 5 days was 50 mg O2/g.

Abiotic control
The abiotic degradation measured as BOD was: 103 % after 28 days.

Key result

Parameter:

BOD5

Value:

50 mg O2/g test mat.

Results with reference substance:

The biodegradation of the reference substance corrected by the blank control and measured as BOD versus ThOD was:
79 % after 7 days
86 % after 14 days
91 % after 28 days

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

FAT 45082/G was found to be not inherently biodegradable.

Executive summary:

The inherent biodegradability of FAT 45082/G was determined in a 28 days Modified MITI (II) - Test according to the OECD Guideline for Testing of Chemicals No 302C (adopted 1981). The test substance and the reference substance were tested in a concentration of about 69.50 mg ThOD/30 mg and 167 mg ThOD/100 mg, respectively. Mean value of the biodegradation of FAT 45082/G after 28 days: 7 % 

Biodegradation of the reference substance after 28 days: 91 %

The biochemical oxygen demand (BOD5) after 5 days was: 50 mg O2/g

BAsed on the present study, it can be concluded that FAT 45082/G was found to be not inherently biodegradable as only 7 % biodegradation was observed after an incubation time of 28 days.

Description of key information

A study was performed to determine the inherent biodegradability of FAT 45082/G in a 28-day Modified MITI (II) - Test according to the OECD Guideline for Testing of Chemicals No. 302C (adopted 1981). The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations. The test substance and the reference substance were tested in a concentration of about 69.50 mg ThOD/30 mg and 167 mg ThOD/100 mg, respectively. Mean value of the biodegradation observed for FAT 45082/G after 28 days was 7 %, whereas biodegradation of the reference substance after 28 days was 91 %. The biochemical oxygen demand (BOD5) after 5 days was 50 mg O2/g. On the basis of the study results FAT 45082/G was found to be not inherently biodegradable after an incubation time of 28 days. Another key study which was carried according to DIN 38409- H 41-1 (1980) corresponding to EEC Directive 92/69/ C.6. (1992) showed COD of 1789 mg O2/g. Vat Black 65 was assessed for biodegradability over the years in the studies estimating BOD5 and COD:

FAT 45082/E- BOD5: 50 -100 mg/g O2; COD: 988.2 mg/g O2

FAT 45082/D- BOD5: 104 mg/g O2; COD: 1723 mg/g O2

FAT 45082/B- BOD5: 14 mg/g O2; COD: 1657 mg/g O2

In all these cases, the BOD5/COD quotient <0.5, therefore the substance is considered to be not readily biodegradable. Taking the data available into account, FAT 45082 is considered to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed