Magnesium hydroxide

Toxicity to reproduction: other studies

Administrative data

Endpoint:

toxicity to reproduction: other studies

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1988

Materials and methods

Principles of method if other than guideline:

The relative cytotoxicity of six metal cations, Cd, Pb, Zn, Ca, Cu and Mg was compared in two primary cell culture systems prepared from rat testes. Cytotoxicity was assayed in Sertoli cell cultures from 21-day old rats after 24-hour incubation with a metal cation.

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

Magnesium oxide

EC Number:

215-171-9

EC Name:

Magnesium oxide

Cas Number:

1309-48-4

Molecular formula:

MgO

IUPAC Name:

Magnesium oxide

Test animals

Species:

rat

Sex:

male

Applicant's summary and conclusion

Conclusions:

Magnesium was without any effect in any assay. Magnesium was not cytotoxic in primary cultures of rat sertoli and testicular tubular cells up to concentrations of 10 mM.

Executive summary:

The testes is a target organ for some toxic metal cations. In this study from Brown et al., 1988 the relative cytotoxicity of six metal cations, Cd, Pb, Zn, Ca, Cu and Mg was compared in two primary cell culture systems prepared from rat testes. Cytotoxicity was assayed in Sertoli cell cultures from 21-day old rats after 24-hour incubation with a metal cation. Cadmium (1 µM), lead (10 µM) and copper (1 mM) inhibited rates of lactate secretions. Cadmium, lead and copper also inhibited reduction of a tetrazolium dye (MTT). Cadmium and copper inhibited the uptake of neutral red by Sertoli cell cultures. Germ cell detachment was assayed in Sertoli germ cell co-cultures from 27 -30-day old rats. Cadmium (1 µM), zinc (0.5 mM) and copper (1mM) stimulated the release of germ cells. Lead did not stimulate the release of germ cells and did not inhibit neutral red uptake. Calcium and magnesium were without any effect in any assay. Although all four of the assays indicated cadmium as very toxic at low concentrations, and calcium and magnesium to be without effect, the results of the assays were not consistent with respect to lead, copper and zinc.