3,5,5-trimethylhexanoyl chloride

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: limited documentation but sufficient for endpoint evaluation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

3 min, 1 h and 4 h testing

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,5,5-trimethylhexanoyl chloride

EC Number:

253-168-4

EC Name:

3,5,5-trimethylhexanoyl chloride

Cas Number:

36727-29-4

Molecular formula:

C9H17ClO

IUPAC Name:

3,5,5-trimethylhexanoyl chloride

Details on test material:

TS-Freetext:
Isononansäurechlorid
- Name of test material (as cited in study report): Isononansaeurechlorid
- Physical state: liquid
- Analytical purity: 98 %

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler
- Weight at study initiation: 2.62 - 3.2 kg

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

other: other flank of the same animal

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): an area of 2x2 cm was coverd

Duration of treatment / exposure:

1. erxeriment 3 min
2. experiment 1 h
3. experiment 4 h

Observation period:

8 days

Number of animals:

2 per experiment

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): mild detergent (lutrol/lutrol 50 %)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #3

Remarks:

4 h exposure

Time point:

24/48/72 h

Max. score:

4

Reversibility:

not specified

Remarks on result:

other: no data for a 3rd animal

Results 2

Irritation parameter:

edema score

Basis:

animal #3

Remarks:

4 h exposure

Time point:

24/48/72 h

Max. score:

4

Reversibility:

not specified

Remarks on result:

other: no data for a 3rd animal

Results 3

Irritation parameter:

erythema score

Basis:

animal #1

Remarks:

4 h exposure

Time point:

24/48 h

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: no value after 72 h; necrosis pergamentous, scars at day 8

Results 4

Irritation parameter:

edema score

Basis:

animal #1

Remarks:

4 hour exposure

Time point:

24/48 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: no value after 72 h

Results 5

Irritation parameter:

erythema score

Basis:

animal #2

Remarks:

4 h exposure

Time point:

24/48 h

Score:

4

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: no value after 72 h; necrosis pergamentous, scars at day 8

Results 6

Irritation parameter:

edema score

Basis:

animal #2

Remarks:

4 h exposure

Time point:

24/48 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: no value after 72 h

Any other information on results incl. tables

Exposition: 4 h

Animal	4 h		24 h		48 h		8 d		Mean (24/48 h)
	Erythema	Edema	Erythema	Edema	Erythema	Edema	Erythema	Edema	Erythema	Edema
1	2 fl	2	3 fl ü	2	3 fl ü	2	4 Np ; Na	2	3	2
2	2 fl	2	4 Nw ü	2	4 Nw	2	4 Np, Na	2	4	2

Exposition: 1 h

Animal	1 h		24 h		48 h		8 d		Mean (24/48 h)
	Erythema	Edema	Erythema	Edema	Erythema	Edema	Erythema	Edema	Erythema	Edema
1	4 Nw	2	4 Nw	2	4 Nw	2	4 Np	2	4	2
2	2	2	3 ü	3 ü	3 ü	3 ü	4 Np	2	3	3

Exposition: 3 min.

Animal	3 min		24 h		48 h		8 d		Mean (24/48 h)
	Erythema	Edema	Erythema	Edema	Erythema	Edema	Erythema	Edema	Erythema	Edema
1	2	0	2 ü	2	2 ü	1	0 Sü	0	2	1.5
2	2 ü	2	3 ü	3	3 ü	3	4 Np	2	3	3
3	2 ü	0	2 ü	2	3 ü	3	0 sS	0	2.5	2.5
4	2 ü	2	2 ü	2	3 ü	3	2 sS	0	2.5	2.5
5	2 ü	2	2 ü	2	2 ü	2	0 sSü	0	2	2
6	2	0	2 ü	2	2 ü	3	0 sSü	0	2	2.5

fl: spotty necrotic (full thickness necrosis)

ü: overlapping

Np: necrosis pergamentous (severe irritation, no full thickness necrosis)

Nw: necrosis soft (severe irritation, no full thickness necrosis)

Na: scars (sign for corrosion)

sS: severe scale formation (sign for irritation if still at end of observation period)

S: scale formation (sign for irritation if still at end of observation period)