3,4-dichlorobenzonitrile

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

June 1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

There is no data available on eye irritation testing with 3,4-Dichlorobenzonitrile. In order to classify the substance according to GHS relevant endpoint data available for 2,6-Dichlorobenzonitrile was read-acrossed to 3,4-Dichlorobenzonitrile based on structural similarities. 2,6-Dichlorobenzonitrile is as well as 3,4-Dichlorobenzonitrile, is a disubstituted Benzonitrile. Both substances only differ in the position of the chloro substituents. Furthermore both substances are solid, practically insoluble in water and have a water octanol partition coefficient of 2.2 and 2.8. This might perhaps also lead to a similiar eye irritation potential of both substances.
For Justifcation of read across see section 13.2

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Dichlobenil

EC Number:

214-787-5

EC Name:

Dichlobenil

Cas Number:

1194-65-6

Molecular formula:

C7H3Cl2N

IUPAC Name:

2,6-dichlorobenzonitrile

Details on test material:

- Name of test material (as cited in study report): 2,6-Dichlorbenzonitril

Test animals / tissue source

Species:

rabbit

Strain:

other: White Russian

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: own breed
- Weight at study initiation: 2.5 - 2.8 kg
- Housing: single in V2A-steelcages with a floor area of 0.4 m2)
- Diet (e.g. ad libitum): KF-G 774 of Laboratorium für Pharmakologie und Toxikologie, 2104 Hamburg 92, Germany) ad libitum
- Water (e.g. ad libitum): tap water ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/- 3 °C
- Humidity (%): 60 +/- 5%
- Photoperiod (hrs dark / hrs light): 12 hours light (300 Lux), 12 hours dark

Test system

Vehicle:

not specified

Controls:

other: right eye served as control

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 mg

Duration of treatment / exposure:

no data

Observation period (in vivo):

before the application, 1, 24 and 72 hours after the apllication

Number of animals or in vitro replicates:

6 rabbits (3 males and 3 females)

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no data
- Time after start of exposure: no data

SCORING SYSTEM:
on the basis of DRAIZE

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

fully reversible

Results 2

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

fully reversible

Results 3

Irritation parameter:

other: Fluorecein Test

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

fully reversible

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

2,6-Dichlorobenzonitrile was tested to be not eye irritatting in a test equivalent to OECD 405.

Executive summary:

2,6 -Dichlorobenzonitrile was tested to be not eye irritation in a test equivalent to OECD 405.

As therer is no data available on eye irritation testing with 3,4 -Dichlorobenzonitrile relevant endpoint data available for 2,6 -Dichlorobenzonitrile was read-acrossed to 3,4 -Dichlorobenzonitrile in order to classify the substance (without any unecessary animal testing) according to GHS. The Read-Across approach was based on structural and physico chemical similarities (see rational for reliability).