Potassium iodide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

In this study the effects of test chemical on the growth and metabolite accumulation of Staphylococcus auerus (JKB068) and Escherichia coli (JKE029).

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Staphylococcus auerus (JKB068), Escherichia coli (JKE029)

Details on inoculum:

Nutrient agar plates containing various concentrations of test chemical were prepared in quintuplicate. The above mentioned organisms were inoculated by standardized loops.

Test type:

not specified

Water media type:

not specified

Total exposure duration:

24 h

Hardness:

No data

Test temperature:

37 deg C

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

No data

Details on test conditions:

No data

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

other: Minimum Inhibitory Concentration (MIC100)

Effect conc.:

358.3 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Staphylococcus auerus

Duration:

24 h

Dose descriptor:

other: Minimum Inhibitory Concentration (MIC100)

Effect conc.:

219.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Effect were observed on Escherichia coli (JKE029)

Details on results:

MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism.

Validity criteria fulfilled:

no

Conclusions:

Minimum Inhibitory Concentration (MIC100) value of test chemical in microorganism (Staphylococcus auerus) in a 24hrs. study on growth effect was determine to be 358.3 ± 17.1 mg/L and Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Escherichia coli) in a 24hrs. study on growth effect was determine to be 219.4 ± 9.6 mg/L.

Executive summary:

In this study the effects of test chemical on the growth and metabolite accumulation of Staphylococcus auerus (JKB068) and Escherichia coli (JKE029). MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism. Minimum Inhibitory Concentration (MIC100) value of test chemical in microorganism (Staphylococcus auerus) in a 24hrs. study on growth effect was determine to be 358.3 ± 17.1 mg/L and Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Escherichia coli) in a 24 hrs study on growth effect was determine to be 219.4 ± 9.6 mg/L.

Description of key information

In this study the effects of test chemical on the growth and metabolite accumulation of Staphylococcus auerus (JKB068) and Escherichia coli (JKE029). MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism. Minimum Inhibitory Concentration (MIC100) value of test chemical in microorganism (Staphylococcus auerus) in a 24hrs. study on growth effect was determine to be 358.3 ± 17.1 mg/L and Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Escherichia coli) in a 24 hrs study on growth effect was determine to be 219.4 ± 9.6 mg/L.

 

Key value for chemical safety assessment

EC50 for microorganisms:

219.4 mg/L

Additional information

Data available for the test chemicals, study has been reviewed to determine the short term toxicity on growth of microorganisms of the test chemical. The studies are as mentioned below:

In the first study from peer reviewed journal the effects of test chemical on the growth and metabolite accumulation of Staphylococcus auerus (JKB068) and Escherichia coli (JKE029). MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism. Minimum Inhibitory Concentration (MIC100) value of test chemical in microorganism (Staphylococcus auerus) in a 24hrs. study on growth effect was determine to be 358.3 ± 17.1 mg/L and Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Escherichia coli) in a 24 hrs study on growth effect was determine to be 219.4 ± 9.6 mg/L.

 

First study was supported by the second study. Aim of this study was to determine the effect of test chemical on the growth of microorganism. Minimum Inhibitory Concentration were checked after the 24 hrs. Nutrient agar plates containing various concentrations of chemical were prepared in quintuplicate. The above mentioned organisms were inoculated by standardized loops. Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Bacillus subitilis) in a 24hrs study on growth effect was determine to be 147.6 ± 10.2mg/L. Thus chemical consider to be nontoxic.

 

Similar study was conducted to determine the effect of test chemical on the growth and mortality of test organisms Clostridium species. Nutrient agar plates containing various concentrations of test chemical were prepared in quintuplicate. The two Clostridium species that had been isolated from the cultures of N. closterium and P. tricornutum, were inoculated by standardized loops. MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism. Test conducted for 24 hrs. Inhibition observed at 231.5 mg/l and 200.9 mg/l on 2nd species.

 

Thus based on the above results, chemical toxicity ranges from 147 mg/l to 231.5 mg/l.