Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reason / purpose for cross-reference:
read-across: supporting information
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.91 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: source substance
Remarks:
partially unsaturated TEA-Esterquat
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.1 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: source substance
Remarks:
partially unsaturated TEA-Esterquat
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
11.23 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: absence of respiratory movement and response to physical movement
Remarks on result:
other: source substance
Remarks:
partially unsaturated TEA-Esterquat
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
5.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: source substance
Remarks:
partially unsaturated TEA-Esterquat
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: source substance
Remarks:
partially unsaturated TEA-Esterquat

Description of key information

96 h LC50 > 100 mg a.i/L, based on analytically verified nominal concentration (OECD TG 236, embryonic stages of Danio rerio); GLP; RL1

96 h LC50 = 1.91 mg a.i/L, based on mean measured concentration (OECD TG 203, Oncorhynchus mykiss); GLP; RL1; read-across: partially unsaturated TEA-Esterquat

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1.91 mg/L

Additional information

No experimental data are available for the target substance Esterification products of triglycerides C18 unsaturated with triethanolamine, dimethyl sulfate quaternized. However, reliable relevant data are available for the closely related source substances partially unsaturated TEA-Esterquat and oleic acid based TEA-Esterquat. A justification for read-across is attached to Iuclid section 13.

 

In a 96-h acute toxicity study conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test), rainbow trouts (Oncorhynchus mykiss) were exposed to partially unsaturated TEA-Esterquat (CAS No. 157905-74-3) at nominal concentrations of 0 (control), 1.0, 1.6, 2.56, 4.1, 6.55, 10.55 mg/L under semi-static conditions. The mean measured concentration was determined to be 59 % of nominal concentrations. The 96-h LC50 was 1.91 mg/L (based on mean measured concentration). Sublethal effects as difficulties with maintainance of balance and unusual behaviour (reduced activity and or orientation to bottom or surface of the test vessels) were observed within the first three hours in the highest test concentration  

 

In a further study performed according to OECD Guideline 203 (Fish, Acute Toxicity Test) under semi-static conditions the LC50 (96 h) of partially unsaturated TEA-Esterquat was determined to be 2.1 mg/L for Danio rerio. The effect concentration (96h-LC50 = 2.6 mg/L) was corrected because the analytical recovery (HPLC) of test substance was below 80% of nominal concentration.

Partially unsaturated TEA-Esterquat was classified as toxic to Danio rerio by the study authors. However, according to CLP (EU-GHS), Regulation (EC) No 1272/2008 the criteria for acute aquatic toxicity of fish (96h LC50) is </= 1 mg/L.

 

There are two further reliable studies available for partially unsaturated TEA-Esterquat performed according to OECD Guideline 203 (Fish, Acute Toxicity Test) under semi-static conditions and a study according to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study) under flow-through conditions, but all performed without analytical control. The LC50 (96 h) effect concentrations were 7.48 mg/L (test mat., nominal, based on a.i. > 80 % the 96h LC50 was > 5.98 mg a.i./L) for Danio rerio and 11.23 mg a.i./L and 14.45 mg a.i./L for Oncorhynchus mykiss each, respectively. The nominal effect concentrations were corrected for the content of active ingredient.

One study is available for Leuciscus idus (Golden orfe). In this study the 24 to 96 h LC 50 of TEA-Esterquat partially unsaturated was 5.1 mg test mat./L (nominal).

In a flow through test according to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study, 1984) the 96 h and 14 d LC50 of partially unsaturated TEA-Esterquat were 4.9 mg/L each (nominal) for Danio rerio.

In an OECD Guideline 203 study under semi-static conditions with oleic acid based TEA-Esterquat the 96 h LC 50 was 4.8 mg/l for rainbow  trout (Oncorhynchus mykiss).  

 

The lowest 96 h LC50 in fish of 1.91 mg/L obtained in rainbow trouts (Oncorhynchus mykiss) will be used for chemical safety assessment.