4,4'-(2,3-epoxypropoxy)-2,2'- dimethyl-5,5'-tert-butyldiphenylsulphide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 September 1998 to 24 December 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

other: Japanese Industrial Standard (JIS K 0102-1993-71) Testing methods for industrial waste water, Acute toxicity test with fish

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Remarks:

HCO-40

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
Test material supplied by the sponsor and HCO-40 (40 times amount of the test material) were dissolved with acetone. After acetone was evaporated from the solution, ion-exchanged water was added to the mixture to prepare 1000 mg/L stock solution.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Orange-red killifish
- The fish were checked visually in the receiving and those demonstrating any abnormalities were removed. The fish were reared for 13 days in a flow through system following an external disinfection.
- Length at study initiation: average 3.0 cm
- Weight at study initiation: average 0.26 g

ACCLIMATION
- After rearing, the fish were transferred to an acclimatising aquarium and acclimatised there after the second external disinfection. The fish those
demonstrating any abnormality were removed and reared for 14 days in a flow through system at the temperature of 25 + 2°C. The fish were checked for health conditions and reared another 62 days after the external disinfection.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

117 mg/L (Ca, Mg)

Test temperature:

25 ± 2°C

pH:

At initial exposure: 7.9
At termination of exposure: 7.6

Dissolved oxygen:

At initial exposure: 8.1 mg/L
At termination of exposure: 7.4 mg/L

Nominal and measured concentrations:

Nominal: 250 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Round glass vessel
- Material, size, headspace, fill volume: 4 L
- Renewal rate of test solution (frequency/flow rate): Renewal of test water, at every 8 - 16 hrs
- No. of organisms per concentration: 10
- No. of organisms per control: 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Underground water from the premises of the testing laboratory was used for the test. The dilution water for test was taken out on 3 August 1998, and it was analysed and measured (once every six months in this laboratory). It was confirmed that the dilution water met the ministerial ordinance of the Ministry of Health and Welfare (December 21, 1992), water quality criteria for fisheries (Shadanhozin Nihon Suisansigen Hogokyokai, March 1983), and OECD Guidelines for Testing of Chemicals 210, Fish, Early-life Stage Toxicity Test (July 17, 1992).

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

The 48-hour LC50 was estimated by Doudoroff method.

Sublethal observations / clinical signs:

Table 1: Cumulative Mortality

Concentration (mg/L)	Cumulative Mortality (%)
	2 hours	24 hours	48 hours
Control	-	0	0
250	-	0	0

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of this study, the 48 hour LC50 value was ≥ 250 mg/L.

Executive summary:

The acute toxicity of the test material to fish was investigated in accordance with Japanese Industrial Standard (JIS K 0102-1993-71.) titled "Testing methods for industrial waste water, Acute toxicity test with fish". The testing was performed under GLP conditions.

Oryzias Iatipes (Orange-red killifish) were exposed to the test material for 48 hours in a semi-static test system. Ten fish were treated at 250 mg/L and ten fish were used as a control group.

The 48-hr LC50 value was estimated by Doudoroff method. No mortality was observed in the test material treated or the control group during the study.

Under the conditions of this study, the 48 hour LC50 value was ≥ 250 mg/L.

Description of key information

Under the conditions of the study, the 48 hour LC50 value was ≥ 250 mg/L.

Additional information

The acute toxicity of the test material to fish was investigated in accordance with Japanese Industrial Standard (JIS K 0102-1993-71.) titled "Testing methods for industrial waste water, Acute toxicity test with fish". The testing was performed under GLP conditions.

Oryzias Iatipes (Orange-red killifish) were exposed to the test material for 48 hours in a semi-static test system. Ten fish were treated at 250 mg/L and ten fish were used as a control group.

The 48-hr LC50 value was estimated by Doudoroff method. No mortality was observed in the test material treated or the control group during the study.

Under the conditions of the study, the 48 hour LC50 value was ≥ 250 mg/L.