Heptyl undec-10-enoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01/2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Heptyl undec-10-enoate

EC Number:

268-850-7

EC Name:

Heptyl undec-10-enoate

Cas Number:

68141-27-5

Molecular formula:

C18H34O2

IUPAC Name:

heptyl undec-10-enoate

Test material form:

liquid

Test solutions

Vehicle:

yes

Remarks:

Carrier solvent used : Carboxymethyl Cellulose (at 30 mg/l concentration)

Details on test solutions:

A limit test was performed at a dose concentration of 100 mg/l of the test item prior to the main study. On the basis of the findings main study was conducted by preparing five concentrations of the test item (i.e. 2, 4, 8, 10, 20 mg/l).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

No. of Daphnia group : 20 Daphnids per concentration (four replicates of asingle
Concentration and each containing Five Daphnids)
Temperature : 18 - 22°C
No. of Dose concentration : 5

Study design

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

21.2-21.6 °C

pH:

6.0-7.2

Dissolved oxygen:

≥ 4 mg/lcontent of the water

Nominal and measured concentrations:

A limit test was performed at a dose concentration of 100 mg/l of the test item prior to the main study. On the basis of the findings main study was conducted by preparing five concentrations of the test item (i.e. 2, 4, 8, 10, 20 mg/l).

Details on test conditions:

16 hours light and 8 hours dark cycle

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.9 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The test and control containers were labeled with Study No., Name of the Product, No. of Daphnia per concentration and the Date of Initiation and Completion of the Experiment. Four replicates of five concentrations i.e 20, 40, 60, 80 and 100 mg/l and 20 Daphnia per concentration (four replicates of single concentration and each replicate containing five Daphnia) was made.

Carrier solvent used : Carboxymethyl Cellulose (at 30 mg/l concentration) no effect
control water : no effect

Reported statistics and error estimates:

EC50 was calculated using Finney’s Probit Analysis

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study i.e. EC50 of Daphnia with “Heptyl Undecylenate” came out as:
EC50 : 3.90 mg/ l of water
95 % F. limit : 2.74- 5.04 mg/l of water
The compound has been tested as per ‘OECD Guideline for the testing of Chemicals, Daphnia Acute
Immobilization Test Guideline No. 202 adopted on 13th April 2004’

Executive summary:

The study was conducted to determine the ‘Immobilization test of ‘Heptyl Undecylenate’ on a benthic zooplankton strain ‘Daphnia magna’. In the present study, a limit test was performed at 100 mg/l concentration, of the test item ‘Heptyl Undecylenate’ with 20 Daphnia (four replicates of five Daphnids each). Based on the result of the limit test, the Main Test was conducted taking five different concentrations of the test item (20, 40, 60, 80 and 100 mg/l). Similarly, 20 Daphnia each (four replicates of five Daphnids each) for negative control (water) and solvent control (at 30mg/l concentration of CMC)) groups were used (the volume of solvent, used in the test concentrations and controls was kept same).

On the basis of these concentrations, the EC50 was estimated which came out as:

EC50 : 3.90mg/l of water

95 % F. limit : 2.74- 5.04 mg/l of water

Lowest concentration causing 100 % immobility in Daphnia : 20 mg/ l of water