N,N-bis(carboxymethyl)glycine, compound with 2-aminoethanol (1:3)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28/02/2005 - 11/04/2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

T

Analytical monitoring:

no

Details on sampling:

- Concentrations: 100, 320, 560, 1000, 1800 mg/L
- Sampling method: Population mortality % measured over time.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test material added by water accommodated fractions
- Controls: Negative control (0 mg/L test material)

Test organisms (species):

other: Scophthalmus maximus

Details on test organisms:

TEST ORGANISM
- Common name: Turbot
- Length at study initiation (length definition, mean, range and SD): 38.6 - 42.2 g
- Weight at study initiation (mean and range, SD): 1.671 - 2.272 g


ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): No
- Type and amount of food during acclimation: pellet food form
- Feeding frequency during acclimation: at least twice a day
- Health during acclimation (any mortality observed): Mortality observations conducted daily

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

Measurements taken at 0, 24, 48, 72 and 96 hours

Test temperature:

13.9-15.1 °C

pH:

6.67-8.11 over 96 hours

Dissolved oxygen:

99-103%

Conductivity:

51.4-55.0 mS/cm

Nominal and measured concentrations:

Nominal concentrations for test substance were 100, 320, 560, 1000, 1800 mg /L

Details on test conditions:

TEST SYSTEM
- Test vessel: soda-lime glass tank
- Material, size, headspace, fill volume: 14 L capacity
- Aeration: gentle aeration
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater from Scapa Flow, Orkney.
- Total organic carbon:
- Particulate matter: Sand filtered to 1 µM
- Alkalinity: pH 7.98
- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS
- Photoperiod: Constant illumination

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality in test population

TEST CONCENTRATIONS
- Test concentrations: Nominal concentrations used were 100, 320, 560, 1000 and 1,800 mg/ L-1

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

other: LC90

Effect conc.:

1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities:
- Mortality of control: Cumulative mortality (0.52%)
- Effect concentrations exceeding solubility of substance in test medium: Effect concentration exceeded maximum concentration tested.

Validity criteria fulfilled:

yes

Conclusions:

The study concludes that the LC50 for 24, 48, 72 and 96 hours was > 1800 mg/L, the LC90 for 96 hours was >1800 mg/L and the NOEC for 96 hours was > 1800 mg/L

Executive summary:

The study assessed the short-term toxicity of Monoethanolamine Salt of Nitrilotriacetic Acid to marine fish. The study was performed in compliance with GLP and in accordance with the testing guidelines OSPARCOM (1995): Protocol for a fish acute toxicity test. The test organisms used in this study was Scophthalmus maximus and all validity criteria were met. The results from the definitive test looked at 5 different concentrations of the test material (100, 320, 560, 1000 and 1800 mg/L) over 4 different time points (24, 48, 72 and 96 hours) with 1 replicate per concentration (7 organisms per replicate). The definitive test concentrations were derived from the allied Skeletonema toxicity test. Monoethanolamine Salt of Nitrilotriacetic Acid exhibited no effect at 1800 mg/L (direct addition) to the marine fish Scophthalmus maximus in the water phase after 96h.

Description of key information

The study assessed the short-term toxicity of Monoethanolamine Salt of Nitrilotriacetic Acid to marine fish. The study was performed in compliance with GLP and in accordance with the testing guidelines OSPARCOM (1995): Protocol for a fish acute toxicity test. The test organisms used in this study was Scophthalmus maximus and all validity criteria were met. The results from the definitive test looked at 5 different concentrations of the test material (100, 320, 560, 1000 and 1800 mg/L) over 4 different time points (24, 48, 72 and 96 hours) with 1 replicate per concentration (7 organisms per replicate). The definitive test concentrations were derived from the Skeletonema toxicity test. Monoethanolamine Salt of Nitrilotriacetic Acid exhibited no effect at 1800 mg/L (direct addition) to the marine fish Scophthalmus maximus in the water phase after 96h.

Key value for chemical safety assessment

Marine water fish

Marine water fish

Effect concentration:

1 800 mg/L