Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

The toxicity to the aquatic environment of N,N'-bis(carboxymethylglycine) compound with 2 -aminoethanol (1:3) was investigated by performing GLP-compliant short-term toxicity studies to algae, fish and aquatic invertebrates. Studies were performed on marine species.

Invertebrates (Acartia Tonsa) was identified as the most sensitive species based on the outcome of the investigation with a 48h LC50 of 1553.85 mg/L. 

The short-term toxicity of the registered substance to aquatic invertebrates was assessed according to ISO 14669 (1999) Water Quality - Determination of acute lethal toxicity to marine copepods (Copepoda; Crustacea) in which test the substance exhibited a 48h LC50 value of 1553.85 mg/L to the marine copepod Acartia tonsa in the aqueous phase.

The short-term toxicity to Algae (Skeletomina Costatum) was investigated in accordance with ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum). N,N'-bis(carboxymethylglycine) compound with 2 -aminoethanol (1:3) showed a 72h EC50 value of 1935.67 mg/L to Skeletonima costatum.

A limit fish test was performed on Scophthalmus maximus with definitive test concentrations derived from the Skeletonema toxicity test. The 96h LC50 obtained was >1800 mg/L.

It can be concluded that the registered does not meet the criteria for classification as toxic to the aquatic environment according to according to Regulation (EC) No.1272/2008 on the Classification, Labelling and Packaging of Substances and Mixtures. 

In accordance with Annex VIII of REACH, Column 2, activated sludge respiration inhibition testing does not need to be conducted if there is no emission to a sewage treatment plant.

Additional information