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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EPA OPP 72-4 (Fish Early Life-Stage and Aquatic Invertebrate Life-Cycle Studies)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-(tert-butyl)-N-sec-butyl-2,6-dinitroaniline
EC Number:
251-607-4
EC Name:
4-(tert-butyl)-N-sec-butyl-2,6-dinitroaniline
Cas Number:
33629-47-9
Molecular formula:
C14H21N3O4
IUPAC Name:
N-sec-butyl-4-tert-butyl-2,6-dinitroaniline
impurity 1
Chemical structure
Reference substance name:
Xylene
EC Number:
215-535-7
EC Name:
Xylene
Cas Number:
1330-20-7
Molecular formula:
C8H10
IUPAC Name:
m-xylene

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
flow-through
Total exposure duration:
21 d

Test conditions

Hardness:
180 mg/L (Total hardness as CaCO3)
Test temperature:
20°C
pH:
7.7 to 9.1
Dissolved oxygen:
7.8 to 8.5 mg/L
Conductivity:
500 µS/cm
Nominal and measured concentrations:
Nominal test concentrations: 19, 38, 75, 150 and 300 µg/L
Mean measured concentrations: 10, 17, 37, 79 and 150 µg a.i./L

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
ca. 130 µg/L
Basis for effect:
mortality
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
ca. 79 µg/L
Basis for effect:
mortality

Applicant's summary and conclusion