Reaction product of 4-aminophenol with 2-ethyl-6-methylbenzenamine, sodium polysulfide and sodium metabisulfite

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

July 27, 1995

Deviations:

yes

Remarks:

For the determination of water solubility about three times the quantity of Test Item necessary to saturate the desired volume of water was applied. This part of the study deviates from the guideline.

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Version / remarks:

19 March 2014

Deviations:

yes

Remarks:

For the determination of water solubility about three times the quantity of Test Item necessary to saturate the desired volume of water was applied. This part of the study deviates from the guideline.

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7840 (Water Solubility)

Version / remarks:

March 1998

Deviations:

yes

Remarks:

For the determination of water solubility about three times the quantity of Test Item necessary to saturate the desired volume of water was applied. This part of the study deviates from the guideline.

GLP compliance:

yes

Type of method:

flask method

Specific details on test material used for the study:

Date of production: 31 May 2017
Expiration date: 31 May 2022

Key result

Water solubility:

13.5 g/L

Conc. based on:

test mat.

Incubation duration:

>= 24 - <= 72 h

Temp.:

20 °C

pH:

>= 10.14 - <= 10.16

 Results of the Method Validation

Measurement repeatability (7 parallels)	CV% < 1 %
Repeatability of the Preparation (n=5/4)	CV% < 1.5 %
Linear range	0.5 - 100 µg/mL
Correlation coefficients	> 0.999
Limit of Quantification	0.5 µg/mL
Limit of Detection	0.2 µg/mL
Recovery from ISO medium	94 %
Recovery from20X AAPmedium	90 %
Recovery from ultra-pure water (1 g/L)	101 %
Recovery from ultra-pure water (12 g/L 15 g/L)*	99 % 88 %
Accuracy(samples used for calibration)	≤ 3 %
Precision	≤ 5 % (10 % at LOQ)
Stability of the test item in ISO medium at the temperature 18-22 °C for 2 days	stable at the test conditions
Stability of the test item in 20X AAP medium at the temperature 22-26 °C for 7 days	43 % at the test conditions
Stock solution stability (0.2 mg/ml)	stable for at least 7 days at 5±3 °C

* performed in this study

Results of the determination of test item concentrations

Sampling time	Measured concentration of test item (g/L)			Measured pH
	Test vessels	Mean with the 95% confidence intervals
After 24h	13.7	13.5 ± 0.35		10.14
	13.5			10.15
	13.4			10.14
After 48h	13.4	13.4 ± 0.13		10.15
	13.5			10.15
	13.4			10.14
After 72h	13.5	13.6 ± 0.67		10.16
	13.3			10.15
	13.9			10.15
Solubility ofthe test item (g/L)		13.5 ± 0.14	-
RSD%		0.4	-
pH of ultra-pure water		-	~7

Conclusions:

The water solubility of the test item was determined to be 13.5 g/L at 20  ± 1 °C using the shake-flask method according to OECD TG 105.

Executive summary:

The water solubility of the test item was determined according to OECD TG 105, EU Method A.6 and EPA OPPTS 830.7840 (Water Solubility) to be 13.5 g/L at 20  ± 1 °C using the shake-flask method for the performance.

Description of key information

The water solubility of the test item was determined to be 13.5 g/L at 20  ± 1 °C using the shake-flask method according to OECD TG 105 and EU Method A.6.

Key value for chemical safety assessment

Water solubility:

13.5 g/L

at the temperature of:

20 °C

Additional information

The water solubility of the test item was determined according to OECD TG 105, EU Method A.6 and EPA OPPTS 830.7840 (Water Solubility) to be 13.5 g/L at 20  ± 1 °C using the shake-flask method for the performance.