Benzenesulfonic acid, para-, monoalkylation products with C14-C18 branched olefins (C15 rich) derived from propene oligomerization, calcium salt, overbased including distillates (petroleum), hydrotreated, solvent-refined, solvent-dewaxed, or catalytic dewaxed, light or heavy paraffinic C15-C50

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The study was performed fully in compliance with the OECD 474 Guideline and according to GLP principles. When used in assessmen to the tested subtsance, the study is considered reliable without restriction according to the criteria of Klimisch, 1997. However, since the study is used in this assessment to evaluate a similar material, the reliability is reduced to reliable with restrictions according to the criteria of Klimisch, 1997.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)

Deviations:

no

GLP compliance:

yes

Type of assay:

micronucleus assay

Test material

Test material information

Constituent 1

Reference substance name:

calcium di(alkyl(C20-C24, even numbered)(branched)-methylbenzenesulfonate containing Distillates (petroleum), hydrotreated heavy paraffinic

Cas Number:

722503-68-6

Molecular formula:

CaS2O6(C54-62)(H94-110)

IUPAC Name:

calcium di(alkyl(C20-C24, even numbered)(branched)-methylbenzenesulfonate containing Distillates (petroleum), hydrotreated heavy paraffinic

Details on test material:

CAS # 722503-68-6 - Benzenesulfonic acid, methyl-, mono-C20-24-branched, alkyl derivs., calcium salts

Test animals

Species:

mouse

Strain:

CD-1

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

Peanut Oil

Details on exposure:

See study report attached

Duration of treatment / exposure:

Single Treatment/exposure

Frequency of treatment:

Single exposure (one-treatment)

Post exposure period:

2 days

Doses / concentrations

Remarks:

Doses / Concentrations:
6 Males / 6 Females per dose level/ of 47.0, 94.0, and 188.0 mg/kg bw; Single IP treatment; 24 harvest/48 hour timepoint
Basis:

No. of animals per sex per dose:

6 males/6 females per dose level/harvest point

Control animals:

yes, concurrent vehicle

Positive control(s):

Cyclophosphamide (CAS 6055-19-2)

Examinations

Tissues and cell types examined:

Bone marrow polychromatic erythrocytes

Details of tissue and slide preparation:

See study report attached

Evaluation criteria:

See study report attached

Statistics:

See study report attached

Results and discussion

Test results

Sex:

male/female

Genotoxicity:

negative

Toxicity:

yes

Vehicle controls validity:

valid

Negative controls validity:

not examined

Positive controls validity:

valid

Additional information on results:

See study report attached

Any other information on results incl. tables

See study report attached

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative
Not Clastogenic

Executive summary:

The objective of this study was to evaluate the test article, {CAS# 722503 -68 -6} for in vivo clastogenic activity and/or disruption of the mitotic apparatus by detecting micronuclei in polychromatic erythrocyte cells (PCEs) in Crl:CD-1^(R) (ICR) BR mouse bone marrow. The test article induced signs of clinical toxicity in the treated animals but was not cytotoxic to the bone marrow (statistically significant decrease in the PCE:NCE ratio). {CAS # 722503 -68 -6} did induce a statistically increase in micronuclei in bone marrow polychronmiatic etythrocytes in the females at the high-dose level; however, the increase was not dose responsive, with within the historic control range, and was, therefore, not considered to be biologically significant. {CAS # 722503 -68 -6} is considered negative in the mouse bone marrow micronucleus test under the conditions of exposure in this assay.