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EC number: 500-263-6
CAS number: 77138-45-5
A study was performed to assess the effect of the test item on the
respiration of activated sewage sludge. The method followed was designed
to be compatible with the OECD Guidelines for Testing of Chemicals
(2010) No. 209 "Activated Sludge, Respiration Inhibition Test (Carbon
and Ammonium Oxidation)".
Following a preliminary range-finding and initial experiment, activated
sewage sludge was exposed to an aqueous dispersion of the test item at
concentrations of 10, 18 and 32 mg/L (5 replicates) for a period of 3
hours at measured temperatures of between 20 and 22 °C with the addition
of a synthetic sewage as a respiratory substrate.
The rate of respiration was determined after 3 hours contact time and
compared to data for the control and a reference item,
The effect of the test item on the respiration of activated sewage
sludge gave a No Observed Effect Concentration (NOEC) after 3 hours
exposure of 18 mg/L.
The reference item gave a 3-Hour EC50 value of 6.6 mg/L, 95% confidence
limits 4.9 to 8.9 mg/L.
The No Observed Effect Concentration (NOEC) after 3 hours exposure was
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