4-ethoxy-2,3-difluoro-4'-propyl-1,1'-biphenyl

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010-11-26 - 2011-04-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: METI Guideline: Concentration Test on Chemical Substances in Fish

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms: days 0, 7, 14, 21, 26, 28

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION: Done according to guideline.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): TWEEN 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)):
Dose 1: Test item: 0.004 mg/L , Vehicle: 0.024 mg/L
Dose 2: Test item: 0.0004 mg/L , Vehicle: 0.0024 mg/L
Control: Test item: --, Vehicle: 0.024 mg/L

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Lot No. 110112
from 2011-01-12
Weight: 3.0 +/- 0.46 g
Length: 6.6 +/- 0.30 cm

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Total depuration duration:

15 d

Hardness:

250 mg/L

Test temperature:

25.0 ° +/- 2.0 °C

pH:

7.8 +/- 0.1

Dissolved oxygen:

7.7 +/- 0.1 mg/L

TOC:

not specified

Salinity:

not specified

Conductivity:

not specified

Details on test conditions:

After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 7, 14, 21, 26 and 28. The analytical values show that the nominal concentrations of 0.004 and 0.0004 mg/L were maintained at about 100 % using TWEEN 80, respectively. At days 0, 7, 14, 21, 26 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted. The extract was analysed with LC.

Nominal and measured concentrations:

nominal concentration 0.004 mg/L:
analytically measured concentrations:
day 0: 0.00320 mg/L
day 7: 0.00348mg/L
day 14: 0.00320 mg/L
day 21: 0.00320 mg/L
day 26: 0.00319 mg/L
day 28: 0.00337 mg/L

nominal concentration 0.0004 mg/L
analytically measured concentrations:
day 0: 0.000322 mg/L
day 7: 0.0000346 mg/L
day 14: 0.000318 mg/L
day 21: 0.000321 mg/L
day 23: 0.000320 mg/L
day 28: 0.000318 mg/L

Reference substance (positive control):

not specified

Details on estimation of bioconcentration:

according to guideline

Conc. / dose:

0.004 mg/L

Temp.:

25 °C

pH:

7.4

Type:

BCF

Value:

2 538 dimensionless

Basis:

normalised lipid fraction

Time of plateau:

7 d

Calculation basis:

steady state

Remarks on result:

other: analytical concentration

Remarks:

Conc.in environment / dose:0.004 mg/L using TWEEN 80 (0.024 mg/l) as solubilizer

Conc. / dose:

0 mg/L

Temp.:

25 °C

pH:

7.4

Type:

BCF

Value:

1 638 dimensionless

Basis:

normalised lipid fraction

Time of plateau:

7 d

Calculation basis:

steady state

Remarks on result:

other: analytical concentration

Remarks:

Conc.in environment / dose:0.0004 mg/L unsing TWEEn 80 (0.0024 mg/L) as solubilizer

Elimination:

no

Parameter:

DT90

Depuration time (DT):

13 d

Details on kinetic parameters:

steady state was reached within 7 days

Metabolites:

none

Details on results:

- Mortality of test organisms: no effects
- Behavioural abnormalities: no effects
- Observations on body length and weight: no effects
- Other biological observations: no effects
- Bound residues forming a plateau: no
- Mortality and/or behavioural abnormalities of control: no
- Loss of test substance during test period: no
- Results with vehicle control: no effects

Validity criteria fulfilled:

yes

Conclusions:

The BCF mean was determined to be 2111 and thus indicates significant bioaccumulation in the test organism.

Executive summary:

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. Two environmental concentrations of the test material of 0.004 and 0.0004 mg/L were used in this study. TWEEN 80 was used as vehicle at concentrations of 0.024 and 0.0024 mg/L. The exposure was maintained during the course of the study at about 80-100 % of the nominal concentrations. The concentrations have been verified analytically using a LC method with UV detection. No signs of toxicity were observed throughout the study. The analysis reveals steady state BCFs of 2538 and 1684 (mean 2111). The BCF is larger than 2000 indicating significant bioaccumulation in the test organism.

Description of key information

No signs of toxicity were observed throughout the study. The analysis reveals steady state BCFs of 2538 and 1684 (mean 2111), indicating significant bioaccumulation in the test organism.

Key value for chemical safety assessment

BCF (aquatic species):

2 111 dimensionless

Additional information

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. Two environmental concentrations of the test material of 0.004 and 0.0004 mg/L were used in this study. TWEEN 80 was used as vehicle at concentrations of 0.024 and 0.0024 mg/L. The exposure was maintained during the course of the study at about 80-100 % of the nominal concentrations. The concentrations have been verified analytically using a LC method with UV detection. No signs of toxicity were observed throughout the study. The analysis reveals steady state BCFs of 2538 and 1684 (mean 2111). The BCF is larger than 2000 indicating significant bioaccumulation in the test organism.