Registration Dossier
Registration Dossier
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EC number: 226-408-0 | CAS number: 5395-50-6
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- pre-guideline study; inhalation hazard assay
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Version / remarks:
- Inhalation hazard test based on H.F. Smyth et al.: Am.Ind.Hyg.Ass.J. 23, 95 - 107 (1962)
- Principles of method if other than guideline:
- - Principle of test: inhalation of an atmosphere enriched with volatile components at 20 °C
- Short description of test conditions: for enrichment, 200 L air/h was conducted through a layer of 5 cm of the product
- Parameters analysed / observed: lethality, clinical signs, necropsy findings - GLP compliance:
- no
- Test type:
- fixed concentration procedure
Test material
- Reference substance name:
- Tetrahydro-1,3,4,6-tetrakis(hydroxymethyl)imidazo[4,5-d]imidazole-2,5(1H,3H)-dione
- EC Number:
- 226-408-0
- EC Name:
- Tetrahydro-1,3,4,6-tetrakis(hydroxymethyl)imidazo[4,5-d]imidazole-2,5(1H,3H)-dione
- Cas Number:
- 5395-50-6
- Molecular formula:
- C8H14N4O6
- IUPAC Name:
- 1,3,4,6-tetrakis(hydroxymethyl)-octahydro-[1,3]diazolo[4,5-d]imidazole-2,5-dione
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male/female
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- not specified
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Source and rate of air: 200 L air/h
- Temperature in air chamber: 20 °C - Duration of exposure:
- 7 h
- Concentrations:
- app. 13mg/L
- No. of animals per sex per dose:
- 12 in total
(3 male and 3 female animals per experiment, 2 repetitions) - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC0
- Effect level:
- >= 13 mg/L air
- Exp. duration:
- 7 h
- Mortality:
- no mortality observed
- Clinical signs:
- other: During exposure: ragged breathing, wiping of snout, nasal secretion Directly after exposure: accelerated breathing, crusted noses in some animals or nasal secretion All signs had ceased by day 1 after exposure.
- Gross pathology:
- no abnormalities detected
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
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