N,N'-methylenediacrylamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-methylenediacrylamide

EC Number:

203-750-9

EC Name:

N,N'-methylenediacrylamide

Cas Number:

110-26-9

Molecular formula:

C7H10N2O2

IUPAC Name:

N,N'-methylenediacrylamide

Test material form:

solid: crystalline

Details on test material:

Purity = 99.8%

Specific details on test material used for the study:

Test item identification : N,N'-Methylenediacrylamide
Batch number : LQ27A1696
Internal number : 2926879-001
Manufacturing date : January 14, 2016
Expiry date : January 13, 2017
Chemical name : N,N'-Methylenediacrylamide
C.A.S. number : 110-26-9
EEC number : 203-750-9
Molecular weight : 154.17 g/mol
Empirical formula : C7H10N2O2
Purity : > 99%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

The test medium was prepared as follows:
prior to the test start, 501 mL of a 100.0 mg test item/L stock solution was prepared by direct weighing into reconstituted water (0.0501 g of test item into 501 mL of reconstituted water).
The obtained solution showed insolubilized flakes of test item, so it was sonicated for about 5 minutes. After sonication, it was clear and transparent.
A negative control with only reconstituted water was also tested.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The study was performed on the freshwater crustacean Daphnia magna Straus. A strain of this species was supplied by MicroBioTests Inc., Belgium in December 2011 (batch number: DM290911).
Since that time, the clone has been bred in the laboratories of the Test Facility under conditions identical to those of the test, with regard to temperature, light and water quality, so that no acclimation was needed prior to testing.
It was daily fed with a fixed amount of the laboratory cultured green algae Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) and with a suspension of the yeast Saccharomyces cerevisiae.
At the test start the organisms used in the test were less than 24 hours old and were not first brood progeny.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

Water Hardness : 140 - 250 mg/L as CaCO3

Test temperature:

The incubation temperature was continuously monitored during the course of the study by a PT100 probe installed in the environmental test chamber. It was in the range 19.96 – 20.04°C with a mean value of 20.00 °C and a standard deviation of 0.02 °C. The temperature range provided by OECD guideline is 20 ± 2°C. The temperature values during the test were within the provided range and did not vary more than 1°C during the test period.

pH:

The pH of the test media after preparation was measured with a pHmeter. pH values were 7.56 for test item solution and 7.43 for negative control. The pH did not vary by more than 0.34 units during the test period, as required in the mentioned guideline.

Dissolved oxygen:

The dissolved oxygen concentration in the test medium and the negative control was measured with a portable oxymeter and it was always higher than 5.0 mg/L according to OECD Guideline No. 202 (2004) that provides a minimum oxygen concentration of 3.0 mg/L.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Effect of potassium dichromate on Daphnia magna:
24 h IC50 = 1.79 mg/L ; Confidence interval (95%) = 1.61 – 2.00 mg/L
Reference data (UNI EN ISO 6341/2004)
24 h IC50 = 0.6 – 2.1

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of N,N'-Methylenediacrylamide was tested in a GLP guideline study on Daphnia magna under static conditions.
The IC0 and the NOEC at 24 and 48 hours were 100 mg/L.