N,N'-methylenediacrylamide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March-April, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

Test guideline 3

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-methylenediacrylamide

EC Number:

203-750-9

EC Name:

N,N'-methylenediacrylamide

Cas Number:

110-26-9

Molecular formula:

C7H10N2O2

IUPAC Name:

N,N'-methylenediacrylamide

Test material form:

solid: crystalline

Details on test material:

Purity = 99.8%

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

The study was performed using aerobic activated sludge from BRIANZACQUE civil wastewater treatment plant. The sludge were collected on March 23, 2016. An homogenized aliquot of the wet sludge was centrifuged, the supernatant was removed while the solid phase was weighed, dried and the ratio of wet to dry weight was calculated to be equal to 10.95 %.

Based on this ratio, calculated amounts of wet sludge was centrifuged, the supernatant was removed and the solid phase was suspended in the test medium to get a concentration equivalent to 3 g dry material per litre.

The prepared sludge inoculum was pre-conditioned to the experimental conditions by aerating it in the dark. The concentrated suspension was used as inoculum to give a final concentration of 15 mg dry material per litre into test flasks.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

50 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

2.1

Sampling time:

28 d

BOD5 / COD results

BOD5 / COD

Key result

Parameter:

ThOD

Value:

1.45 mg O2/g test mat.

Remarks on result:

other: THOD without nitrification

Results with reference substance:

Theoretical Oxygen Demand without nitrification process) was calculated to be equal to 1.67 mg O2/mg for reference item, Sodium Benzoate.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item, N,N'-Methylenediacrylamide was found to be not ready and completely biodegradable under the conditions applied in a manometric respirometry test, having showed a maximum biodegradation of 2.1% during the test period (mean percentage values between two replicates) at the end of the test period.No significant degradation was observed in the abiotic control.

Moreover, the test item showed no inhibitory effects on the microorganism activity at the tested concentration, as demonstrated by the biodegradation percentage in the toxicity control exceeding the 25% pass level after three days of exposure (30.3%).