Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 217-978-1 | CAS number: 2028-63-9
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The influence of the test substance on the growth of the freshwater algae Scenedesmus subspicatus (new name: Desmodesmus subspicatus) was assessed in a static dose response test according to DIN 38 412. Based on act. ingr. the 72h-ErC50 value was calculated to be 196.88 mg/L (extrapolated), and the associated 72h-ErC10 was determined to be 56.77 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 196.88 mg/L
- EC10 or NOEC for freshwater algae:
- 56.77 mg/L
Additional information
The toxicity of the test substance was determined according to the principles of DIN 38412 Part L9. The aim of the study was to assess the effects on growth rate and yield over a period of 72 and 96 hours. The study was conducted under static conditions. This study encompassed 6 treatment groups (5 dose rates of the test item and control) with one replicate per test concentration and one replicate for the control. At test start 50 mL of the test concentrations were inoculated with 10000 algal cells per mL test medium and defined volumes of the algal suspensions were sampled after 0, 24, 48, 72 and 96 hours for determination of cell densities by spectrophotometrical measurement. No analytical determination of the concentrations of the test substance was done. The nominal test concentrations were 0.5, 5, 50 and 500 mg test item/L.
Based on act. ingr., the 72h-ErC50 was determined to be 196.88 mg test item/L and the associated 72h-ErC10 was determined to be 56.77 mg test item/L. The 72h-EyC10 was determined to be 50.92 mg/L and the 72h-EyC50 was determined to be 170.92 mg/L
Also based on act. ingr., the 96h-ErC50 was determined to be 272.34 mg/L and the associated 96h-ErC10 was determined to be 3.89 mg/L. The associated 96h-EyC10 was determined to be 35.06 mg/L and the associated 96h-EyC50 was determined to be 103.76 mg/L. All values were based on nominal concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
