Reaction products of sodium sulphide, sulphur, carbon disulphide and potassium hydroxide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

March 22, 1991 - July 3, 1991

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

No analytical monitoring and study did not account for degradation to carbon disulphide and subsequent losses resulting from the volatilization of carbon disulphide from the test system.

Qualifier:

according to guideline

Guideline:

other: DIN 38 412

Version / remarks:

DEV L15

Deviations:

yes

Remarks:

There was no analytical monitoring or account for degradation to carbon disulphide and subsequent loss of carbon disluphide through volatilization.

GLP compliance:

yes

Specific details on test material used for the study:

A red-orange liquid, recieved on April 22, 1991 in a 100 mL polyethylene bottle.

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The dilution water used was synthetic.

Test organisms (species):

Leuciscus idus

Details on test organisms:

The organism used was a fresh-water fish species obtained from Gebr. De Boon B.V. They were acclimized to labratory conditions for 19 days, and were not fed 48 hours before the begining of the test.

Test type:

static

Water media type:

freshwater

Remarks:

Synthetic freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

The dead animals were counted and removed after 24hr and 48 hr. At the same times the condition of the survivors were visually compared with that of the control animals.

Test temperature:

The temperature of the control solution was 19.8 deg C; 20.3 deg C; and 20.7 deg C

pH:

Between 7.3 and 8.4.

Dissolved oxygen:

The lowest value measured was 6.0 mg/L^-1

Nominal and measured concentrations:

32 mg/l; 56 mg/l; 100 mg/l; 180 mg/l, and 320 mg/l

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

55 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

There was no mortality in the control solution

Validity criteria fulfilled:

no

Remarks:

No analytical monitoring and no consideration given to the degradation of substance to carbon disulphide and losses of carbon disulphide due to volatilization.

Conclusions:

The acute toxicity (48-hr LC50) of Amersep MP7 to freshwater fish species Leuciscus idus (L.) was determined to be 55 mg/l.

Executive summary:

In an acute toxicity study conducted according to DIN 38 412, DEV L15 and OECD principles for GLP, the acute toxicity (48-hr LC50) of Amersep MP7 to freshwater fish species Leuciscus idus (L.) was determined to be 55 mg/l. However, significant deficiencies in the study design exist. Specifically, there was no analytical monitoring and there was no consideration given to the degradation of the substance to carbon disulphide and losses of carbon disluphide due to volatilization.

Description of key information

In an acute toxicity study conducted according to DIN 38 412, DEV L15 and OECD principles for GLP,the acute toxicity (48-hr LC50) of Amersep MP7 to freshwater fish species Leuciscus idus (L.) was determined to be 55 mg/l. However, significant deficiencies in the study design exist. Specifically, there was no analytical monitoring and there was no consideration given to the degradation of the substance to carbon disulphide and losses of carbon disluphide due to volatilization.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

55 mg/L