Estr-4-ene-3,17-dione

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples for the concentration analysis were taken at time 0 (measurement vessel) and after 48 hours (all test vessels, pooled) after start of exposure.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM:
- Specification: Daphnia magna Straus, Cladocera, Phyllopoda
- Source: Schering AG, Institute for Experimental Toxicology
- Age: 0-25.5 hours
- Holding: Parent animals were kept in glass vessels in water of the same quality and temperature as under experimental conditions; continuous feeding with a laboratory culture of microalgae, e.g. Chlorella vulgaris

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

277.52 mg/L (CaCO3)

Test temperature:

20.8 to 21.4 °C

pH:

7.7-8.5

Dissolved oxygen:

6.9 and 9.1 mg/L

Salinity:

n.a.

Nominal and measured concentrations:

The nominal concentrations of the test solutions were 6.2, 12.5, 25, 50 and 100 mg/L. Samples tor the concentration analysis by HPLC in combination with UV detection were taken at start of exposure and 48 hours thereafter. The analytical results gave concentrations of 96.6, 48.0, 24.9, 12.5 and 6.2 mg/L.

Details on test conditions:

I. Preparation of the stock solution and the test concentration:
- 111 mg test substance were suspended in 1000 ml tap water under constant stirring for 24 hours and filtered through a glassfibre filter and was used as the highest test concentration
- Aliquots of the solution were diluted 1:2, 1:4, 1:8 and 1:16 with tap water in order to prepare the different test concentrations

II. EXPOSURE CONDITIONS:
- Test vessels: LabelIed 100 ml test vessels holding 45 ml of the test medium or tap water and 5 ml of the dilution water with the daphnids
- Experimental design: 4 replicates each of the test solution and the dilution water control with 5 Daphnia in each vessel; 1 vessel without daphnids for each test and control solution, for the measurements of pH, temperature and oxygen content
- Method of initiation: neonates were placed in prepared media
- Photoperiod: 16 h light : 8 h dark
- Aeration: none
- Feeding: None
- Test item concentration: 6.2, 12.5, 25, 50 and 100 mg/L
- Medium renewal: none
- Criteria of effects: Immobilization and visual abnormalities were recorded at 24 and 48 hours

III. Hydrographical measurements:
- One additional vessel without Daphnia (measurement vessel) for each test and control solution, respectively, was used for the measurements of pH, temperature and oxygen content
- pH, oxygen concentration and temperature were measured at 0 and 48 hours

IV. Observations:
- Immobilization and visual abnormalities were recorded at 24 and 48 hours

V. Statistical analysis:
- The EC50 was calculated by the computer programme "Probit-Analysis", Institute of Biometrics, Schering AG, Berlin (Germany)

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

16.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 14.1-19.2 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The 48 h-EC50 to Daphnia magna was calculated to be 16.3 mg/L (95% confidence limits (14.1-19.2)) on the basis of measured concentrations.

Executive summary:

The purpose of this study was to determine the acute toxicity with Daphnia magna in order to evaluate the aquatic toxicity. The study was conducted as a definitive test in agreement with the test guideline of the OECD, no. 202. Twenty juvenile Daphnia magna were used tor each test solution of the test substance and twenty tor the tap water control. The Daphnia were exposed for aperiod of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. For the preparation of the test solutions a suspension with a nominal concentration of 100 mg/I was stirred tor 24 hours. This suspension was filtered through a glassfibre filter. The resulting solution served as the highest concentration. It was further diluted 1:2, 1:4, 1:8 and 1:16. The nominal concentrations of the test solutions were 6.2, 12.5, 25, 50 and 100 mg/L. Samples tor the concentration analysis by HPLC in combination with UV detection were taken at start of exposure and 48 hours thereafter. The analytical results gave concentrations of 96.6, 48.0, 24.9, 12.5 and 6.2 mg/L. Statistical evaluation regarding the observations of immobilization was performed by probit analysis. Immobilized Daphnia were observed in the test solutions containing 12.5, 24.9, 48 and 96.6 mg/L. The highest concentration without effects was 6.2 mg/L. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute daphnia study.

Description of key information

The 48 h-EC50 to Daphnia magna was calculated to be 16.3 mg/L (95% confidence limits (14.1-19.2)) on the basis of measured concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

16.3 mg/L