Hexasodium 4,4'-[vinylenebis[(3-sulphonato-4,1-phenylene)imino[6-morpholino-1,3,5-triazine-4,2-diyl]imino]]bis[5-hydroxy-6-(phenylazo)naphthalene-2,7-disulphonate]

Short-term toxicity to fish

Description of key information

The 96-h LC50 value of the test substance (88% purity) was greater than 100 mg/L (based on nominal concentrations, corrected for purity).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Key study

The acute toxicity of the test item (88% purity) to fish (zebrafish) was determined according to the OECD TG 203 (1992) and EU Method C.1 (2008). A static limit test with one application at the test start was conducted with a test item concentration of 114 mg/L, corresponding to 100 mg/L, corrected for purity. The test item amount was weighed and added directly to the water in the test vessel. The solution was mixed with an ultraturrax (1 min, 17000 rpm). The test duration was 96 hours. 7 test organisms each were exposed to the test item solution and the control. The water quality parameters temperature, pH-value and oxygen saturation measured at 0, 24, 48, 72 and 96 hours were within the acceptable limits. The concentrations of the test item in the limit test concentration and in the control were analytically verified via spectrophotometric analysis at the start and end of the exposure (0 and 96 hours). At the start of the exposure (0 hours), the measured concentration of the test item, corrected for purity, was 96 mg/L for the test item solution. At the end of exposure (96 hours), the value was 97 mg/L. There was no test item found in the control samples either at the start or the end of the exposure period. Therefore, all effect values were based on the nominal concentration of the test item, corrected for purity. The LC50 after 2, 24, 48, 72 and 96 -h was > 100 mg/L, corrected for purity. The 96 -h LC100 value was > 100 mg/L and the 96 -h LC0 value was 100 mg/L. In conclusion, the test item was found to cause no toxic effects to zebrafish after 96 hours when tested with a test item solution of 100 mg/L.

Supporting study

An acute fish toxicity study according to the OECD guideline 203 was performed under static conditions. Zebra fish (ten per concentration) were treated with 100.0, 300.0 and 1000.0 mg/L of the test material (60% act. ingredient) for 1, 4, 24, 48, 72 and 96 hours. The LC50 value is greater than 1000 mg/L 48 hours and 96 hours.