Ammonium nitrate

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian germ cell study: cytogenicity / chromosome aberration

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

Not a guideline study. The information given is limited to the above mentioned. The aberration frequency at 5.2 mg/kg bw is without biological meaning and may be a mistake in the table. It is not clear whether a metaphase-arresting agent was added before sacrifice.

Data source

Reference

Reference Type:

publication

Title:

Evaluation of the mutagenic activity of ammonium nitrate and dimethylamine salt of 2,4-dichlorophenoxyacetic acid

Author:

Nechkina, MA

Year:

1992

Bibliographic source:

Gigiena i sanitariia (2); p. 66-7

Materials and methods

Principles of method if other than guideline:

Only one sex used, sampling earlier than 1.5 normal cell cycle; no positive controls.

GLP compliance:

not specified

Type of assay:

chromosome aberration assay

Test material

Test material information

Constituent 1

Reference substance name:

Ammonium nitrate

EC Number:

229-347-8

EC Name:

Ammonium nitrate

Cas Number:

6484-52-2

Molecular formula:

H3N.HNO3

IUPAC Name:

ammonium nitrate

Test material form:

solid

Remarks:

granules or crystals

Details on test material:

No further details provided.

Test animals

Species:

mouse

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

- Sex: male
- Age: sexually-mature
- Number: 6/dose
- Controls: 9 animals vehicle only

Administration / exposure

Route of administration:

oral: gavage

Duration of treatment / exposure:

15 days

Frequency of treatment:

daily

Post exposure period:

Sampling times and number of samples: 6 h after last administration

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

0 mg/kg bw (total dose)

Doses / concentrations 2

Dose / conc.:

5.2 mg/kg bw (total dose)

Doses / concentrations 3

Dose / conc.:

20.9 mg/kg bw (total dose)

Doses / concentrations 4

Dose / conc.:

104 mg/kg bw (total dose)

Doses / concentrations 5

Dose / conc.:

417 mg/kg bw (total dose)

No. of animals per sex per dose:

6/dose

Control animals:

other: 9 animals vehicle only (vehicle = water)

Examinations

Tissues and cell types examined:

Chromosome preprations were prepared using the standard Ford method from bone marrow cells; 100 metaphases were examined per animal.

Results and discussion

Test results

Key result

Sex:

male

Genotoxicity:

negative

Remarks:

No increase in aberrations was found in the treated groups relative to the negative control.

Additional information on results:

EFFECT ON MITOTIC INDEX OR PCE/NCE RATIO: not indicated

GENOTOXIC EFFECTS: No increase in aberrations was found in the treated groups relative to the negative control.

ABERRATION FREQUENCY:
- Number of metaphases with aberrations (gaps excluded): -0.5, 0.3, 0.7, 0.8 and 1.1 per 100 metaphases for 0, 5.2, 20.9, 104 and 417 mg/kg bw, respectively