Sodium 3-nitrobenzenesulphonate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Justification for type of information:

Data is from experimental report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

A Draize Test was carried out for chemical Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4) to assess the eye irritation potential of six white Vienna rabbits.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 3-nitrobenzenesulphonate

EC Number:

204-857-3

EC Name:

Sodium 3-nitrobenzenesulphonate

Cas Number:

127-68-4

Molecular formula:

C6H5NO5S.Na

IUPAC Name:

sodium 3-nitrobenzenesulphonate

Test material form:

solid

Details on test material:

Name of the test chemical: Sodium 3-nitrobenzenesulfonate
Molecular Formula:C6H5NO5S.Na
Molecular Weight: 225.1556 g/mol
Substance Type: Organic
Physical State: Solid

Specific details on test material used for the study:

Name of the test chemical: Sodium 3-nitrobenzenesulfonate
Molecular Formula:C6H5NO5S.Na
Molecular Weight: 225.1556 g/mol
Substance Type: Organic
Physical State: Solid

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

Mean body weight of the rabbits: 2.7 kg for males and 2.64 kg for females

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eye of the same animal

Amount / concentration applied:

Amount applied: 0.1 ml

Duration of treatment / exposure:

24 h

Observation period (in vivo):

8 d

Number of animals or in vitro replicates:

6

Details on study design:

The eyes were not washed out after 24 hours after substance application. Findings were graded as described in OECD test guideline 405.
Findings were recorded after 24, 48, 78 hours, and at the end of the observation period (8 days).

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

48 h

Score:

0.3

Max. score:

1

Reversibility:

not reversible

Remarks on result:

positive indication of irritation

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

48 h

Score:

0.2

Max. score:

1

Reversibility:

fully reversible

Remarks on result:

probability of weak irritation

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 48 h

Score:

0.5

Max. score:

2

Reversibility:

fully reversible within: 8 d (exemption: reddening)

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

24 h after application of the test substance, 2/6 animals showed opacicity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 6/6 animals showed redness of the conjunctivae grade 2, 5/6 showed swelling of the conjunctivae grade 1 and 6/6 showed secretion. 48 h after application of the test substance, 2/6 animals showed opacicity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 4/6 showed secretion. 72 h after application of the test substance, 2/6 animals showed opacicity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 3/6 showed secretion.
Opacicity of the cornea (grade 1, area 1/4-1/2) persisted in 2/6 animals while conjunctival redness grade 1 persisted in 3/3 animals until the end of the observation period. Other signs of toxicity were not noted.

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

From the experimental results, it is concluded that sodium 3-nitrobenzenesulphonate is regarded as irritating to the eye of white Vienna rabbits.

Executive summary:

A Draize Test was carried out for chemical Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4) to assess the eye irritation potential of six white Vienna rabbits.

About 0.1ml of test chemical was instilled into the eye of each rabbits and the eyes were not washed out after 24 hours after substance application.

 Findings were graded as described in OECD test guideline 405 and were recorded after 24, 48, 78 hours, and at the end of the observation period (8 days).

24 h after application of the test substance, 2/6 animals showed opacicity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 6/6 animals showed redness of the conjunctivae grade 2, 5/6 showed swelling of the conjunctivae grade 1 and 6/6 showed secretion. 48 h after application of the test substance, 2/6 animals showed opacity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 4/6 showed secretion. 72 h after application of the test substance, 2/6 animals showed opacity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 3/6 showed secretion. Opacity of the cornea (grade 1, area 1/4-1/2) persisted in 2/6 animals while conjunctival redness grade 1 persisted in 3/3 animals until the end of the observation period. Other signs of toxicity were not noted.

Thus from the observed findings, it is concluded that the chemical Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4) was irritating to the eyes of white Vienna rabbits.