Reaction mass of pentasodium 2-{[4-chloro-6-(ethyl{3-[(2-sulfonatoethyl)sulfonyl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-5-hydroxy-6-({2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl}diazenyl)naphthalene-1,7-disulfonate and tetrasodium 2-[(4-chloro-6-{ethyl[3-(vinylsulfonyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-5-hydroxy-6-({2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl}diazenyl)naphthalene-1,7-disulfonate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 February 2006 to 03 April 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Specific details on test material used for the study:

Identity: FAT 40824/A
Batch: Red ROE 805 BOP 04/05
Appearance: dark red powder
Purity: Organic part (Na-salt): approx. 82 %; Main component 1: approx. 36.2 %; Main component 2: approx. 27.5 %; Oligomers: 10 %
Expiration date: 01 October 2010
Storage: At room temperature at about 20 °C in a desiccator (hygroscopic)

Water solubility:

>= 373 g/L

Remarks on result:

other: room temperature

Details on results:

After mixing a total of about 2.5 g of test item in 5 ml of water a dark coloured solution was obtained. It was scarily possible to decide by visual judgement if complete dissolution was achieved. The water solubility of FAT 40824/A might therefore be higher as determined in this study. The determination of the concentration in the above mentioned test item/water mixture was done by HPLC with respect to a calibration curve. A main test was not performed.

Table : Results obtained for the solubility of FAT 40824/A in water

Initial Weight of Test Item [g]	Water added [ml]	Dilution Factor	Measured Concentration [mg/l]	Solubility in water [g/l]	Mean [g/l]
2.91	5	20000	20.103	402.05	372.79
2.04	5	20000	17.176	343.53

 

Conclusions:

The water solubility of FAT 40824/A was found to be at least 373 g/l (or above) at room temperature.

Executive summary:

The determination of the water solubility of FAT 40824/A was performed according to the EEC Directive 92/69, A.6 "Water Solubility" (1992) and the OECD Guideline No. 105 "Water Solubility" (1995) using a simplified flask method. After mixing a total of about 2.5 g of test item in 5 ml of water a dark coloured solution was obtained. It was scarily possible to decide by visual judgement if complete dissolution was achieved. The water solubility of FAT 40824/A might therefore be higher as determined in this study. The determination of the concentration in the above mentioned test item/water mixture was done by HPLC with respect to a calibration curve. A main test was not performed. In conclusion, the water solubility of FAT 40824/A was found to be at least 373 g/l (or above) at room temperature.

Description of key information

The water solubility of the test substance is ≥373 g/l.

Key value for chemical safety assessment

Water solubility:

373 g/L

at the temperature of:

20 °C

Additional information

In a GLP-compliant study, the determination of the water solubility of FAT 40824/A was performed according to the EEC Directive 92/69, A.6 "Water Solubility" (1992) and the OECD Guideline No. 105 "Water Solubility" (1995) using a simplified flask method. After mixing a total of about 2.5 g of test item in 5 ml of water a dark coloured solution was obtained. It was scarily possible to decide by visual judgement if complete dissolution was achieved. The water solubility of FAT 40824/A might therefore be higher as determined in this study. The determination of the concentration in the above mentioned test item/water mixture was done by HPLC with respect to a calibration curve. A main test was not performed. In conclusion, the water solubility of FAT 40824/A was found to be at least 373 g/l (or above) at room temperature.