Registration Dossier
Registration Dossier
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EC number: 231-977-3 | CAS number: 7783-06-4
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
In vitro point mutation assay with bacteriae:
With H2S one Ames-Test with only limited reliability exists. This test showed a negative result. This is confirmed by the result of a fully reliable Ames-test with Na2S showing a negative result in the Salmonella typhimurium strains TA 1535, TA 1537, TA 98, and TA 100 (Engelhardt. 1989).
In vitro point mutation with mammalian cells:
HPRT-test (hypoxanthine-guanine phosphoribosyl transferase (hprt) locus) with L5178Y
mouse lymphoma cells showed a negative result.
In vivo cytogenetic/chromosomal aberration mutation assay:
In a Dominant-lethal-test Wistar rats were exposed to H2S on 7 days for 3 hours per day at a concentration of 220 ppm (306 mg H2S/m³). The study gave no indication on a genetic toxicity of H2S.
The ability of sodium sulphide to produce micronuclei in the bone marrow cells of NMRI mice was evaluated following two i.p. injections (Gocke et al., 1981). No significant effects were observed at dosages that ranged from 24 to 96.1 mg/kg.
The result gave no indication for a mutagenic activity.Justification for selection of genetic toxicity endpoint
None selected, negative battery of genotoxicity assays
Short description of key information:
In an aqueous environment H2S and its salts NaHS and Na2S dissolve easily and are immediately hydrolyzed and an pH-dependent equilibrium is established between S2-, HS- and H2S. Therefore Na2S can be used to evaluate the mutagenic potential of H2S. So the evaluation of the genetic toxicity of H2S is based on the availiable studies with H2S supplemented by studies with Na2S.
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
According to REGULATION (EC) No 1272-2008 and Annex VI of Commission Directive 2001/59/EC/ Council Directive 67/548 EEC, H2S is not classified for germ cell mutation.
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