2'-anilino-6'-[ethyl(3-methylbutyl)amino]-3'-methylspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 20, 1981 to February 27, 1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study undertaken to recognised guidelines but no confirmation of GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: in vivo fish bioconcentration study guidelines referenced in Kanpogyo Notification No. 5, Yakuhatsu Notification No. 615 and 49 Kikyoku Notification No. 392

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-anilino-3-methyl-6-(N-ethyl-N-3-methylbutyl) aminofluoran

IUPAC Name:

2-anilino-3-methyl-6-(N-ethyl-N-3-methylbutyl) aminofluoran

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): 2-anilino-3-methyl-6-(N-ethyl-N-3-methylbutyl) aminofluoran
- Substance type:Heat sensitive powder used in printing.
- Physical state: light yellowish pink powder
- Analytical purity: 98%
- Lot/batch No.: S-560

Radiolabelling:

no

Test solutions

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dispersing agent: hydrogenated castor oil and olive oil.

Dispersion method: The test substance was dissolved in acetone together with 80 volumes of castor oil and 20 volumes of olive oil. After removing acetone, the solution obtained was diluted with water to prepare a dispersion of 1,000 ppm (w/v).

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Japanese rice fish
- Length at study initiation (mean): 9.8cm
- Weight at study initiation (mean): 23.4g

ACCLIMATION
- Acclimation period: 25°C for 14 days
- Acclimation conditions: same as test

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

30 d

Test conditions

Test temperature:

25±1°C

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tank
- Type: open
- Material, fill volume: glass, 100 L
- Aeration:
- Type of flow-through: peristaltic
- Renewal rate of test solution (flow rate):597 l / day
- No. of organisms per vessel: 6
- No. of vessels per concentration (replicates):1

Nominal and measured concentrations:

Nominal; level 1 0.5 and level 2 0.05 ppm w/v

Reference substance (positive control):

no

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

30 dimensionless

Basis:

whole body d.w.

Calculation basis:

kinetic

Remarks on result:

other: Level 1

Remarks:

Conc.in environment / dose:0.05 ppm

Bioaccumulation factor 2

Type:

BCF

Value:

396 dimensionless

Basis:

whole body d.w.

Calculation basis:

kinetic

Remarks on result:

other: Level 2

Remarks:

Conc.in environment / dose:0.5 ppm

Details on results:

- Mortality of test organisms: nil
- Behavioural abnormalities: nil
- Observations on weight 30g:

Reported statistics:

Level 1 (0.5ppm) conc in water tank (ppm) 0.494, 0.494, 0.494, 0.494, 0.494, 0.479, 0.479; concentration in fish (ppm) 11.8, 10.4, 12.1, 23.9, 5.09, 24.4 and 15.8.,

BCF, 23.9, 21.0, 24.5, 48.3, 10.3, 50.9, 33.0. mean BCF = 30.27

Mean weight, 21.16g mean conc in fish, 14.78 ppm


Level 2 (0.05 ppm) conc in water tank (ppm) 0.0498, 0.0498, 0.0503, 0.0503, 0.0507, 0.0507. concentration in fish (ppm) 15.5, 12.0, 26.5, 21.6, 20.5 and 41.8 (ppm)

BCF, 311, 240, 526, 430, 404, 825 mean BCF = 396

Applicant's summary and conclusion

Conclusions:

The BCF resulting from this study range from 30 to 396 which are considered low. Therefore, this suggest that this substance does not haver a tendency to bioaccumulate.