Benzoic acid, 2-amino-5-fluoro-, methyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and Guideline study with no or minor deficiencies in documentation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

314-24-4

IUPAC Name:

314-24-4

Constituent 2

Reference substance name:

methyl 2-amino-5-fluorobenzoate

EC Number:

608-681-1

Cas Number:

319-24-4

Molecular formula:

C8 H8 F N O2

IUPAC Name:

methyl 2-amino-5-fluorobenzoate

Test material form:

other: yellow solid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

The starting solution was prepared as follows: 128 mg of the test substance were dissolved in 800 ml of water and stirred for 3 h at 20°C and a pH of 7.8. Then the solution was filled up to 1 l. The test concentrations have been prepared by dilution of this starting solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
Common name: Daphnia Magna Straus
Strain: Klon 5
Source:Daphnienzucht der Hoechst AG, Abteilung UWS
Age at study initiation (mean and range, SD): 6-24 h
Method of breeding: The animals were kept in petri dishes (ca. 4 l). Breeding media M4 according to Elendt

Feeding during test:
Food type: Algae Scenedesmus subspicatus
Frequency: 2 per day

Study design

Test type:

static

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 °C

pH:

7.8

Dissolved oxygen:

8.7-9.0 mg/l

Nominal and measured concentrations:

0(crtl), 16, 22.63, 32, 45.26, 64, 90.51, 128 mg/l nominal

Details on test conditions:

20 animals were kept in a beaker per concentration group ( in duplicates). After 24 hours and 48 hours the activity of the animals wasa tested.

Reference substance (positive control):

yes

Remarks:

Potassiumdichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

26.91 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

26.91 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of the test item is 10 mg/L- 100 mg/L und the given test conditions.