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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study according to EU / OECD guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
methyl 4-cyano-2-nitrobenzoate
EC Number:
610-840-5
Cas Number:
52449-76-0
Molecular formula:
C9H6N2O4
IUPAC Name:
methyl 4-cyano-2-nitrobenzoate

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
nominal hardness of 0.24 mmol Ca+Mg/L
Test temperature:
Nominally 23 ± 2 °C
Nominal and measured concentrations:
nominal: 0.156 - 0.313 - 0.625 - 1.25 - 2.5 - 5 - 10 mg/L
mean measured: 0.079 - 0.200 - 0.500 - 1.08 - 2.26 - 4.52 - 8.86 mg/L
Reference substance (positive control):
yes
Remarks:
Potassium dichromate p.a.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
other: EbC50
Effect conc.:
0.691 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
other: Inhibition of biomass growth
Remarks on result:
other: 0.655 - 0.729 mg/L
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
1.08 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: Inhibition of biomass growth
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.5 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: Inhibition of biomass growth
Duration:
72 h
Dose descriptor:
other: ErC50
Effect conc.:
1.16 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: Rate-related inhibition
Remarks on result:
other: 1.12 - 1.20
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
1.08 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: Rate-related inhibition
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.5 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: Rate-related inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In this study the test item was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus at mean measured test item
concentrations ≥ 1.08 mg/L (biomass growth and growth rate). The EC50-values with 95 % confidence intervals for inhibition of biomass growth
(EbC50) and specific growth rate (ErC50) after 72 h were 0.691 (0.655 - 0.729) and 1.16 (1.12 - 1.20) mg/L, respectively. Inhibition effects were
found to be reversible up to 8.86 mg/L, the highest tested concentration. All effect levels are given based on mean measured concentrations of
the test item.