Registration Dossier
Registration Dossier
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EC number: 610-840-5 | CAS number: 52449-76-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to EU / OECD guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- methyl 4-cyano-2-nitrobenzoate
- EC Number:
- 610-840-5
- Cas Number:
- 52449-76-0
- Molecular formula:
- C9H6N2O4
- IUPAC Name:
- methyl 4-cyano-2-nitrobenzoate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 35 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
Reference substance
- Reference substance:
- acetic acid, sodium salt
- Remarks:
- functional control
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 1
- Sampling time:
- 3 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 2
- Sampling time:
- 7 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 4
- Sampling time:
- 14 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 5
- Sampling time:
- 21 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 6
- Sampling time:
- 28 d
- Details on results:
- The test item in the test concentration of 35 mg/L did not reach the Ievel of 10 % (begin of the biodegradation).
The biodegradation remained at a Ievel of 0-6 %.
The pass Ievel of a biodegradation > 70 % was not reached neither in the 1 0-d-window nor after
28 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The test item must be regarded to be not readily biodegradable in the 1 0-d-window and after 28 days.
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