Reaction mass of benzyldodecylbis(2-hydroxypropyl)ammonium chloride and benzylbis(2-hydroxypropyl)tetradecylammonium chloride

Short-term toxicity to fish

Description of key information

Brachydanio rerio were exposed under static conditions for 96 h. No mortality or abnormalities were observed at a test concentration of 1.1 mg/L. 

Additional information

No explanation is provided for the fact that 100 % mortality was observed testing 1.6 mg/L, but only behavioural effects occured at a concentration of 2.2 mg/L. Therefore, the study was scored as not reliable. Nevertheless, results were used in the scope of the hazard assessment as they provide evidence that no effect will be observed below a concentation of 1.1 mg/L. Results of these tests will be used in the scope of thechemical safety assessment (PNEC-derivation),classification and labellingas well as thePBT-assessment.

With respect to thePNEC-derivationin the scope of the chemical safety assessment, daphnids were shown to be the most sensitive aquatic species with an EC50 value of 0.97 mg/L forming the basis for the PNEC derivation. Nevertheless, results of the tests with algae, daphnids and fish provide evidence, that adverse effects towards aquatic organsims are to be found in the same order of magnitude (around a concentration of 1 mg/L). There is no evidence that fish will be more sensitive than daphnids further lowering the derived PNEC value significantly.

With respect toclassification and labelling, daphnids were shown to be the most sensitive aquatic species triggering the most stringent classification with respect to environmental hazards. Results of a new fish test would not result in a new, more stringent classification of the substance with respect to environmental hazards.

ThePBT-assessmentof a substance is commonly based on environmental and toxicological results. As the substance already fulfills the T-criterion based on its toxicological profile, results of a new fish test would not change that assessment. No new information would be obtained through testing.

Concluding, no new study will be conducted as the risk for the aquatic compartmant is adequately described based on the already available information and no new information would be obtained through testing.