3-icosyl-4-henicosylidene-2-oxetanone

Sediment toxicity

Administrative data

Endpoint:

sediment toxicity: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 225

Deviations:

no

Principles of method if other than guideline:

OECD 225 rather than OECD 217 or 218 was chosen as PMC D-532 logKow is >5.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-icosyl-4-henicosylidene-2-oxetanone

EC Number:

401-210-9

EC Name:

3-icosyl-4-henicosylidene-2-oxetanone

Cas Number:

83708-14-9

Molecular formula:

C44H84 O2

IUPAC Name:

(4Z)-4-henicosylidene-3-icosyloxetan-2-one

Details on test material:

Hercules identification: PMC D-532
Batch number: 27DN519
Storage conditions: room temperature in the dark

Sampling and analysis

Analytical monitoring:

yes

Test substrate

Vehicle:

yes

Test organisms

Test organisms (species):

Lumbriculus variegatus

Study design

Study type:

laboratory study

Test type:

static

Water media type:

freshwater

Type of sediment:

artificial sediment

Limit test:

yes

Exposure duration

Duration:

28 d

Exposure phase:

total exposure duration

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 3

Duration:

28 d

Dose descriptor:

LOEC

Effect conc.:

> 1 000 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50(28d) for reproduction based on nominal test concentrations was greater than 1000 mg/kg. The NOEC was 1000 mg/kg and the LOEC was greater than 1000 mg/kg.

Executive summary:

A study was performed to assess the toxicity of the test material PMC D-532 on reproduction to the sediment-dwelling oligocheate, Lumbriculus variegatus. The method was based on the OECD Guideline No. 225 "Sediment-water Lumbriculus toxicity Test using Spiked Sediment.

4 replicates of 10 worms were exposed to formulated sediment spiked with test material at concentrations of 10, 32, 100, 320 and 1000 mg/kg (dw sediment) for a period of 28 days. Control and solvent control groups (both 6 x 10 worms) were maintained under identical conditions but not exposed to the test material. Worms exposed to pentachlorophenol sodium salt served as positive control. The numbers of worms and the dry weight data of these worms were recorded at the end of the test.

No significant effects were observed at any of the concentrations tested. The EC50(28d) for reproduction based on nominal test concentrations was greater than 1000 mg/kg. The NOEC was 1000 mg/kg and the LOEC was greater than 1000 mg/kg.