4-amino-2,5-dichloro-N,N-dimethylbenzenesulphonamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study conducted in accordance with "Good Scientific Practice"

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

no

Remarks:

but in accordance with the principles of "Good Scientific Practice"

Test material

Test material information

Constituent 1

Reference substance name:

4-amino-2,5-dichloro-N,N-dimethylbenzenesulphonamide

EC Number:

247-501-2

EC Name:

4-amino-2,5-dichloro-N,N-dimethylbenzenesulphonamide

Cas Number:

26175-68-8

Molecular formula:

C8H10Cl2N2O2S

IUPAC Name:

4-amino-2,5-dichloro-N,N-dimethylbenzene-1-sulfonamide

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna (Straus, 1820); breeding: parental and young Daphnia were held in 250 l glas aquaria
Ilumination: 16 hours a day
temperature: 20 +-2 °C
medium: continously aerated tap water from Zürich
Feed: boiled supernatant of a 1 : 1 mixture of Sera micropan and Sera vipan (Sera apanristic GmbH, D-5138 heinsberg) prepared with tap water (once a day)
Test vessel: 100 ml flasks, all-glass with 50 ml of test medium
Number of daphnia: 40 individuals per concentration, 20 per vessel

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

21 +- 0.5 °C

pH:

8.5 - 8.6

Dissolved oxygen:

7.4 - 7.9 mg/L

Nominal and measured concentrations:

Nominal: 0.6, 1.0, 1.9, 3.4, 6.2, 11.1, 20.0 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

other: % immobility: 22.5

Effect conc.:

0.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

other: % immobilty: 52.5

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

48 h

Dose descriptor:

other: % immobility: 60

Effect conc.:

1.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 4

Duration:

48 h

Dose descriptor:

other: % immobility: 70

Effect conc.:

3.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 5

Duration:

48 h

Dose descriptor:

other: % immobility: 77.5

Effect conc.:

6.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 6

Duration:

48 h

Dose descriptor:

other: % immobility: 80

Effect conc.:

11.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 7

Duration:

48 h

Dose descriptor:

other: % immobility: 97.5

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 was determined as 1.42 mg/L for toxicity against daphnia

Executive summary:

40 individual daphnia devided in 2 test vessels were exposed each to 20.0, 11.1, 6.2, 3.4, 1.9, 1.0 and 0.6 mg/L. based on the observed mortality after 48 hours the EC50 value of the test item was calculated as 1.42 mg/L. (EC0=0.09 mg/L; EC100=23.0 mg/L)